Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae
文献类型：期刊论文
| 作者 | Zhang, Jian; Chen, Min3; Dong, Xiaoyu4; Lin, Ruozhu1; Fan, Jianhua; Chen, Zhiduan
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| 刊名 | PLOS ONE
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| 出版日期 | 2015 |
| 卷号 | 10期号:5 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0125574 |
| 文献子类 | Article |
| 英文摘要 | Many species of Schisandraceae are used in traditional Chinese medicine and are faced with contamination and substitution risks due to inaccurate identification. Here, we investigated the discriminatory power of four commonly used DNA barcoding loci (ITS, trnH-psbA, matK, and rbcL) and corresponding multi-locus combinations for 135 individuals from 33 species of Schisandraceae, using distance-, tree-, similarity-, and character-based methods, at both the family level and the genus level. Our results showed that the two spacer regions (ITS and trnH-psbA) possess higher species-resolving power than the two coding regions (matK and rbcL). The degree of species resolution increased with most of the multilocus combinations. Furthermore, our results implied that the best DNA barcode for the species discrimination at the family level might not always be the most suitable one at the genus level. Here we propose the combination of ITS+trnH-psbA+matK+rbcL as the most ideal DNA barcode for discriminating the medicinal plants of Schisandra and Kadsura, and the combination of ITS+trnH-psbA as the most suitable barcode for Illicium species. In addition, the closely related species Schisandra rubriflora Rehder & E. H. Wilson and Schisandra grandifloraHook.f. & Thomson, were paraphyletic with each other on phylogenetic trees, suggesting that they should not be distinct species. Furthermore, the samples of these two species from the southern Hengduan Mountains region formed a distinct cluster that was separated from the samples of other regions, implying the presence of cryptic diversity. The feasibility of DNA barcodes for identification of geographical authenticity was also verified here. The database and paradigm that we provide in this study could be used as reference for the authentication of traditional Chinese medicinal plants utilizing DNA barcoding. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | MOLECULAR PHYLOGENETICS ; CHEMICAL-CONSTITUENTS ; COMMUNITY PHYLOGENY ; MAXIMUM-LIKELIHOOD ; TRNH-PSBA ; IDENTIFICATION ; RBCL ; BIODIVERSITY ; SEQUENCES ; CHINENSIS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000353943000077 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of China [31100171, 31270268] ; Major Innovation Program of Chinese Academy of Sciences [KSCX2-EW-Z-2] ; National Basic Research Program of China [2014CB954101] ; CAS International Research & Education Development Program [SAJC201315] ; CAS External Cooperation Program of BIC [GJ II Z201321] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26015]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Jiangsu Prov & Chinese Acad Sci, Nanjing Bot Garden Mem Sun Yat Sen, Inst Bot, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Chen, Min,Dong, Xiaoyu,et al. Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae[J]. PLOS ONE,2015,10(5). |
| APA | Zhang, Jian,Chen, Min,Dong, Xiaoyu,Lin, Ruozhu,Fan, Jianhua,&Chen, Zhiduan.(2015).Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae.PLOS ONE,10(5). |
| MLA | Zhang, Jian,et al."Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae".PLOS ONE 10.5(2015). |
入库方式： OAI收割
来源：植物研究所
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