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ycf1, the most promising plastid DNA barcode of land plants
文献类型：期刊论文
| 作者 | Dong, Wenpan; Xu, Chao; Li, Changhao3; Sun, Jiahui3; Zuo, Yunjuan; Shi, Shuo; Cheng, Tao; Guo, Junjie2; Zhou, Shiliang |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015 |
| 卷号 | 5 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep08348 |
| 文献子类 | Article |
| 英文摘要 | A DNA barcode is a DNA fragment used to identify species. For land plants, DNA fragments of plastid genome could be the primary consideration. Unfortunately, most of the plastid candidate barcodes lack species-level resolution. The identification of DNA barcodes of high resolution at species level is critical to the success of DNA barcoding in plants. We searched the available plastid genomes for the most variable regions and tested the best candidates using both a large number of tree species and seven well-sampled plant groups. Two regions of the plastid gene ycf1, ycf1a and ycf1b, were the most variable loci that were better than existing plastid candidate barcodes and can serve as a barcode of land plants. Primers were designed for the amplification of these regions, and the PCR success of these primers ranged from 82.80% to 98.17%. Of 420 tree species, 357 species could be distinguished using ycf1b, which was slightly better than the combination of matK and rbcL. For the well-sampled representative plant groups, ycf1b generally performed better than any of the matK, rbcL and trnH-psbA. We concluded that ycf1a or ycf1b is the most variable plastid genome region and can serve as a core barcode of land plants. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | BERLIN |
| WOS关键词 | CHLOROPLAST GENOME ; MATK PRIMERS ; PHYLOGENY ; GENE ; BIOGEOGRAPHY ; LEPECHINIA ; EVOLUTION ; RADIATION ; CLADE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000349245100001 |
| 出版者 | NATURE PORTFOLIO |
| 资助机构 | Ministry of Science and Technology of China [2011FY120200, 2012BAC01B05, 2012AA021602] ; National Natural Science Foundation of China [31270239] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26021]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Wenpan,Xu, Chao,Li, Changhao,et al. ycf1, the most promising plastid DNA barcode of land plants[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Dong, Wenpan.,Xu, Chao.,Li, Changhao.,Sun, Jiahui.,Zuo, Yunjuan.,...&Zhou, Shiliang.(2015).ycf1, the most promising plastid DNA barcode of land plants.SCIENTIFIC REPORTS,5. |
| MLA | Dong, Wenpan,et al."ycf1, the most promising plastid DNA barcode of land plants".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：植物研究所
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