中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia

文献类型:期刊论文

作者Xu, Songzhi; Li, Dezhu2; Li, Jianwu3; Xiang, Xiaoguo; Jin, Weitao; Huang, Weichang4; Jin, Xiaohua; Huang, Luqi1
刊名PLOS ONE
出版日期2015
卷号10期号:1
ISSN号1932-6203
DOI10.1371/journal.pone.0115168
文献子类Article
英文摘要DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides, Dendrobium is a notoriously difficult group to identify. DNA barcoding was expected to be a supplementary means for species identification, conservation and future studies in Dendrobium. We assessed the power of 11 candidate barcodes on the basis of 1,698 accessions of 184 Dendrobium species obtained primarily from mainland Asia. Our results indicated that five single barcodes, i.e., ITS, ITS2, matK, rbcL and trnH-psbA, can be easily amplified and sequenced with the currently established primers. Four barcodes, ITS, ITS2, ITS+matK, and ITS2+matK, have distinct barcoding gaps. ITS+matK was the optimal barcode based on all evaluation methods. Furthermore, the efficiency of ITS+matK was verified in four other large genera including Ficus, Lysimachia, Paphiopedilum, and Pedicularis in this study. Therefore, we tentatively recommend the combination of ITS+ matK as a core DNA barcode for large flowering plant genera.
学科主题Multidisciplinary Sciences
出版地SAN FRANCISCO
WOS关键词INTERNAL TRANSCRIBED SPACER ; PHYLOGENETIC ANALYSIS ; SYSTEMATICS ; IDENTIFICATION ; DISCRIMINATE ; SEQUENCES ; TAXONOMY ; RATES ; MATK ; LOCI
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000348203500009
出版者PUBLIC LIBRARY SCIENCE
资助机构National Natural Science Foundation of China [31107176, 31470299, J1310002] ; Chinese Special Fund for Medicine Research in the Public Interest [201407003]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26023]  
专题植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文
作者单位1.Shanghai Chenshan Bot Garden, Shanghai 201602, Peoples R China
2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China
4.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Menglun Township 666303, Yunnan, Peoples R China
5.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China
推荐引用方式
GB/T 7714
Xu, Songzhi,Li, Dezhu,Li, Jianwu,et al. Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia[J]. PLOS ONE,2015,10(1).
APA Xu, Songzhi.,Li, Dezhu.,Li, Jianwu.,Xiang, Xiaoguo.,Jin, Weitao.,...&Huang, Luqi.(2015).Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia.PLOS ONE,10(1).
MLA Xu, Songzhi,et al."Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia".PLOS ONE 10.1(2015).

入库方式: OAI收割

来源:植物研究所

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