Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia
文献类型：期刊论文
| 作者 | Xu, Songzhi; Li, Dezhu2; Li, Jianwu3; Xiang, Xiaoguo; Jin, Weitao; Huang, Weichang4; Jin, Xiaohua ; Huang, Luqi1
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| 刊名 | PLOS ONE
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| 出版日期 | 2015 |
| 卷号 | 10期号:1 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0115168 |
| 文献子类 | Article |
| 英文摘要 | DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides, Dendrobium is a notoriously difficult group to identify. DNA barcoding was expected to be a supplementary means for species identification, conservation and future studies in Dendrobium. We assessed the power of 11 candidate barcodes on the basis of 1,698 accessions of 184 Dendrobium species obtained primarily from mainland Asia. Our results indicated that five single barcodes, i.e., ITS, ITS2, matK, rbcL and trnH-psbA, can be easily amplified and sequenced with the currently established primers. Four barcodes, ITS, ITS2, ITS+matK, and ITS2+matK, have distinct barcoding gaps. ITS+matK was the optimal barcode based on all evaluation methods. Furthermore, the efficiency of ITS+matK was verified in four other large genera including Ficus, Lysimachia, Paphiopedilum, and Pedicularis in this study. Therefore, we tentatively recommend the combination of ITS+ matK as a core DNA barcode for large flowering plant genera. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | INTERNAL TRANSCRIBED SPACER ; PHYLOGENETIC ANALYSIS ; SYSTEMATICS ; IDENTIFICATION ; DISCRIMINATE ; SEQUENCES ; TAXONOMY ; RATES ; MATK ; LOCI |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000348203500009 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of China [31107176, 31470299, J1310002] ; Chinese Special Fund for Medicine Research in the Public Interest [201407003] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26023]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Shanghai Chenshan Bot Garden, Shanghai 201602, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Menglun Township 666303, Yunnan, Peoples R China 5.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Songzhi,Li, Dezhu,Li, Jianwu,et al. Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia[J]. PLOS ONE,2015,10(1). |
| APA | Xu, Songzhi.,Li, Dezhu.,Li, Jianwu.,Xiang, Xiaoguo.,Jin, Weitao.,...&Huang, Luqi.(2015).Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia.PLOS ONE,10(1). |
| MLA | Xu, Songzhi,et al."Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia".PLOS ONE 10.1(2015). |
入库方式： OAI收割
来源：植物研究所
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