Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils
文献类型：期刊论文
| 作者 | Chen, Zhe1; Wang, Changhui4; Gschwendtner, Silvia2; Willibald, Georg; Unteregelsbacher, Sebastian; Lu, Haiyan; Kolar, Allison; Schloter, Michael2; Butterbach-Bahl, Klaus; Dannenmann, Michael |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 87页码:67-77 |
| 关键词 | N2O and N-2 cnorB and nosZ Denitrification DNRA Climate change Montane grassland |
| ISSN号 | 0038-0717 |
| DOI | 10.1016/j.soilbio.2015.03.030 |
| 文献子类 | Article |
| 英文摘要 | The montane grassland soils of Europe store significant amounts of nitrogen (N), and climate change might drive their volatilization due to the stimulation of gaseous nitrous oxide (N2O) and dinitrogen (N-2) losses. Hence, a thorough, mechanistic understanding of the processes responsible for N loss and retention such as denitrification and dissimilatory nitrate reduction to ammonium (DNRA) in these soils is urgently needed. Here we aimed to explore the relationships between denitrifier gene abundance and expression with N-2 and N2O production and the importance of DNRA versus denitrification in nitrate consumption and N2O production for typical montane grassland soils of Southern Germany. In a laboratory incubation experiment with glucose and nitrate addition, we combined direct measurements of N2O and N-2 production with a molecular analysis of the denitrifier communities involved in nitrite, nitric oxide (NO) and N2O reduction and with the quantification of DNRA. The soils originated from a space-for-time climate change experiment, where intact plant-soil mesocosms were exposed for three years either to ambient conditions at a high elevation site (HE control treatment) or to predicted climate change conditions (warming, reduced summer precipitation and reduced winter snow cover) by translocation to lower elevation (LE climate change treatment). The abundance (DNA) of cnorB genes was significantly reduced in LE soils, whereas the abundance of nosZ genes did not differ between the HE and LE soils. However, the decreased abundance of cnorB genes unexpectedly resulted in slightly increased rather than decreased potential N2O emissions. This effect could be explained by the increased levels of cnorB mRNA and, therefore, the higher physiological activity of the NO reducers in the LE soils. In contrast with the DNA levels, the dynamics of the cnorB mRNA levels followed N2O emission patterns, whereas the nosZ expression was strongly correlated with the N-2 emission (R-2 = 0.83). The potential rates of DNRA were approximately one-third of the rates of denitrification, and DNRA was not a source for N2O. We conclude that DNRA significantly competes with denitrification in these soils, thus contributing to N conservation. This work demonstrates that the molecular analysis of nosZ gene expression has great potential to contribute to solving the enigmatic problem of understanding N-2 loss from soil. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Soil Science |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1879-3428 |
| WOS关键词 | MESSENGER-RNA LEVELS ; DENITRIFYING BACTERIA ; ACETYLENE INHIBITION ; MICROBIAL COMMUNITY ; RIBOSOMAL-RNA ; N2O EMISSIONS ; NOSZ GENES ; ABUNDANCE ; NIRK ; QUANTIFICATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000357244300008 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | Alexander von Humboldt Foundation [1148319] ; terrestrial ecosystem observatory initiative (TERENO) by BMBF ; Helmholtz Society ; Bavarian government within the FORKAST project ; National Natural Science Foundation of China [21107109] ; German Science Foundation (DFG) [DA 1217/2-1, SCHL 446/11-1] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25874]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Karlsruhe Inst Technol, Inst Meteorol & Climate Res, Atmospher Environm Res IMK IFU, D-82467 Garmisch Partenkirchen, Germany 2.Chinese Acad Sci IBCAS, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 3.German Res Ctr Environm Hlth GmbH, Res Unit Environm Genom, D-85764 Neuherberg, Germany 4.Chinese Acad Sci, Inst Atmospher Phys, State Key Lab Atmospher Boundary Layer Phys & Atm, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhe,Wang, Changhui,Gschwendtner, Silvia,et al. Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils[J]. SOIL BIOLOGY & BIOCHEMISTRY,2015,87:67-77. |
| APA | Chen, Zhe.,Wang, Changhui.,Gschwendtner, Silvia.,Willibald, Georg.,Unteregelsbacher, Sebastian.,...&Dannenmann, Michael.(2015).Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils.SOIL BIOLOGY & BIOCHEMISTRY,87,67-77. |
| MLA | Chen, Zhe,et al."Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils".SOIL BIOLOGY & BIOCHEMISTRY 87(2015):67-77. |
入库方式： OAI收割
来源：植物研究所
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