A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea
文献类型：期刊论文
| 作者 | Liu, Yongbo; Zhang, Ying-Xue; Song, Song-Quan; Li, Junsheng; Stewart, C. Neal, Jr.; Wei, Wei4; Zhao, Yujie; Wang, Wei-Qing |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015 |
| 卷号 | 5 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep15480 |
| 文献子类 | Article |
| 英文摘要 | Transgene insertions might have unintended side effects on the transgenic host, both crop and hybrids with wild relatives that harbor transgenes. We employed proteomic approaches to assess protein abundance changes in seeds from Bt-transgenic oilseed rape (Brassica napus) and its hybrids with wild mustard (B. juncea). A total of 24, 15 and 34 protein spots matching to 23, 13 and 31 unique genes were identified that changed at least 1.5 fold (p < 0.05, Student's t-test) in abundance between transgenic (tBN) and non-transgenic (BN) oilseed rape, between hybrids of B. juncea (BJ) x tBN (BJtBN) and BJ x BN (BJBN) and between BJBN and BJ, respectively. Eight proteins had higher abundance in tBN than in BN. None of these proteins was toxic or nutritionally harmful to human health, which is not surprising since the seeds are not known to produce toxic proteins. Protein spots varying in abundance between BJtBN and BJBN seeds were the same or homologous to those in the respective parents. None of the differentially-accumulated proteins between BJtBN and BJBN were identical to those between tBN and BN. Results indicated that unintended effects resulted from transgene flow fell within the range of natural variability of hybridization and those found in the native host proteomes. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | BERLIN |
| WOS关键词 | MEDICAGO-TRUNCATULA SEEDS ; HEAT-STABLE PROTEOME ; SUBSTANTIAL EQUIVALENCE ; UNINTENDED CHANGES ; CROP ALLELES ; PROTEINS ; GENE ; INTROGRESSION ; EXPRESSION ; PLANTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000363123000001 |
| 出版者 | NATURE RESEARCH |
| 资助机构 | Natural Science Foundation of China [31200288] ; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309038] ; one Project of State Key Laboratory of Environmental Criteria and Risk Assessment [SKLECRA2013OFP14] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25965]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Henan Univ, Coll Chem & Chem Engn, Kaifeng 475001, Peoples R China 2.Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 5.Chinese Acad Sci, State Key Lab Vegetat & Environm Change, Inst Bot, Beijing 100093, Peoples R China 6.Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA |
| 推荐引用方式 GB/T 7714 | Liu, Yongbo,Zhang, Ying-Xue,Song, Song-Quan,et al. A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Liu, Yongbo.,Zhang, Ying-Xue.,Song, Song-Quan.,Li, Junsheng.,Stewart, C. Neal, Jr..,...&Wang, Wei-Qing.(2015).A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea.SCIENTIFIC REPORTS,5. |
| MLA | Liu, Yongbo,et al."A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：植物研究所
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