Integrated and comparative proteomics of high-oil and high-protein soybean seeds
文献类型:期刊论文
作者 | Xu, Xiu Ping1; Liu, Hui3; Tian, Lihong; Dong, Xiang Bai; Shen, Shi Hua3; Qu, Le Qing |
刊名 | FOOD CHEMISTRY
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出版日期 | 2015 |
卷号 | 172页码:105-116 |
关键词 | Antioxidant Comparative analysis Global protein expression Proteomics Soybean |
ISSN号 | 0308-8146 |
DOI | 10.1016/j.foodchem.2014.09.035 |
文献子类 | Article |
英文摘要 | We analysed the global protein expression in seeds of a high-oil soybean cultivar (Jiyu 73, JY73) by proteomics. More than 700 protein spots were detected and 363 protein spots were successfully identified. Comparison of the protein profile of JY73 with that of a high-protein cultivar (Zhonghuang 13, ZH13) revealed 40 differentially expressed proteins, including oil synthesis, redox/stress, hydrolysis and storage-related proteins. All redox/stress proteins were less or not expressed in JY73, whereas the expression of the major storage proteins, nitrogen and carbon metabolism-related proteins was higher in ZH13. Biochemical analysis of JY73 revealed that it was in a low oxidation state, with a high content of polyunsaturated fatty acids and vitamin E. Vitamin E was more active than antioxidant enzymes and protected the soybean seed in a lower oxidation state. The characteristics of high oil and high protein in soybean, we revealed, might provide a reference for soybean nutrition and soybean breeding. (C) 2014 Elsevier Ltd. All rights reserved. |
学科主题 | Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics |
出版地 | OXFORD |
电子版国际标准刊号 | 1873-7072 |
WOS关键词 | CYTOSOLIC GLUTAMINE-SYNTHETASE ; STORAGE PROTEINS ; LIPID-PEROXIDATION ; BETA-CONGLYCININ ; GLYCININ ; ACCUMULATION ; EXPRESSION ; SEQUENCE ; MOBILIZATION ; GERMINATION |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
WOS记录号 | WOS:000345207200015 |
出版者 | ELSEVIER SCI LTD |
资助机构 | National Program of Transgenic Variety Development of China [2014ZX08012-002] |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25575] ![]() |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Xiu Ping,Liu, Hui,Tian, Lihong,et al. Integrated and comparative proteomics of high-oil and high-protein soybean seeds[J]. FOOD CHEMISTRY,2015,172:105-116. |
APA | Xu, Xiu Ping,Liu, Hui,Tian, Lihong,Dong, Xiang Bai,Shen, Shi Hua,&Qu, Le Qing.(2015).Integrated and comparative proteomics of high-oil and high-protein soybean seeds.FOOD CHEMISTRY,172,105-116. |
MLA | Xu, Xiu Ping,et al."Integrated and comparative proteomics of high-oil and high-protein soybean seeds".FOOD CHEMISTRY 172(2015):105-116. |
入库方式: OAI收割
来源:植物研究所
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