Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma
文献类型：期刊论文
| 作者 | Hao, He; Pu, Gao Bin2; Lei, Cai Yan1; Ye, He Chun3; Wang, Hong |
| 刊名 | BIOLOGIA
 |
| 出版日期 | 2015 |
| 卷号 | 70期号:8页码:1053-1062 |
| 关键词 | Arnebia euchroma callus shikonin derivatives ammonium ion gene expression |
| ISSN号 | 0006-3088 |
| DOI | 10.1515/biolog-2015-0122 |
| 文献子类 | Article |
| 英文摘要 | Callus cultures of Arnebia euchroma (Royle) Johnst can produce considerable amounts of shikonin derivatives, which are shown to possess a wide spectrum of pharmaceutical properties, such as antimicrobial, anticancer, antioxidative, immune regulative and anti-inflammatory. To explore the possible regulation mechanism of shikonin derivatives biosynthesis, the effects of ammonium ion (NH4+) on cell growth and formation of shikonin derivatives in callus cultures of A. euchroma were investigated in this paper. The main results include: When A. euchroma callus were cultured in medium with different concentrations of NH4+ (0 mM, 7.02 mM, 14.04 mM), the fresh weight of the cell cultured in 7.02 mM NH4+ was higher than those in 0 mM and 14.04 mM NH4+, indicating that NH4+ is necessary for the cell growth of A. euchroma callus cultures, while excessive NH4+ concentration (14.04 mM) could rather inhibit the cell growth of A. euchroma. The quantitative analysis of the seven main naphthoquinone compounds showed that the contents of most of the shikonin derivatives (including the bioactive components acetylshikonin and beta,beta-dimethyl-acrylshikonin) of the Red Strain cultured in 0 mM NH4+ condition were higher than those in other NH4+ conditions (7.02 mM, 14.04 mM) at most of the sample points, suggesting that NH4+ essentially plays an unfavorable role in the biosynthesis of shikonin derivatives. The analysis of quantitative real-time RTPCR showed that a higher NH4+ concentration inhibited the expression of key enzyme genes (such as AePGT) of shikonin derivatives biosynthesis, while -NH4+ condition was conducive to the expression of such genes. In sum, our results show that NH4+ is necessary for the cell growth of A. euchroma, while unnecessary, or even adversary to the biosynthesis of shikonin derivatives. |
| 学科主题 | Biology |
| 出版地 | BERLIN |
| 电子版国际标准刊号 | 1336-9563 |
| WOS关键词 | LITHOSPERMUM-ERYTHRORHIZON ; GENE-EXPRESSION ; CDNA CLONING ; CULTURES ; PROTEIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000361772300008 |
| 出版者 | WALTER DE GRUYTER GMBH |
| 资助机构 | National Natural Science Foundation of China [61173098, 61379081] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25576]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Shandong Univ Tradit Chinese Med, Jinan 250022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, He,Pu, Gao Bin,Lei, Cai Yan,et al. Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma[J]. BIOLOGIA,2015,70(8):1053-1062. |
| APA | Hao, He,Pu, Gao Bin,Lei, Cai Yan,Ye, He Chun,&Wang, Hong.(2015).Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma.BIOLOGIA,70(8),1053-1062. |
| MLA | Hao, He,et al."Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma".BIOLOGIA 70.8(2015):1053-1062. |
入库方式： OAI收割
来源：植物研究所
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