Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth
文献类型：期刊论文
| 作者 | Zhou, Zhenzhen; Shi, Haifan; Chen, Binqing; Zhang, Ruihui2; Huang, Shanjin2; Fu, Ying |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2015 |
| 卷号 | 27期号:4页码:1140-1161 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.114.135400 |
| 文献子类 | Article |
| 英文摘要 | Pollen tubes deliver sperms to the ovule for fertilization via tip growth. The rapid turnover of F-actin in pollen tube tips plays an important role in this process. In this study, we demonstrate that Arabidopsis thaliana RIC1, a member of the ROP-interactive CRIB motif-containing protein family, regulates pollen tube growth via its F-actin severing activity. Knockout of RIC1 enhanced pollen tube elongation, while overexpression of RIC1 dramatically reduced tube growth. Pharmacological analysis indicated that RIC1 affected F-actin dynamics in pollen tubes. In vitro biochemical assays revealed that RIC1 directly bound and severed F-actin in the presence of Ca2+ in addition to interfering with F-actin turnover by capping F-actin at the barbed ends. In vivo, RIC1 localized primarily to the apical plasma membrane (PM) of pollen tubes. The level of RIC1 at the apical PM oscillated during pollen tube growth. The frequency of F-actin severing at the apex was notably decreased in ric1-1 pollen tubes but was increased in pollen tubes overexpressing RIC1. We propose that RIC1 regulates F-actin dynamics at the apical PM as well as the cytosol by severing F-actin and capping the barbed ends in the cytoplasm, establishing a novel mechanism that underlies the regulation of pollen tube growth. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-298X |
| WOS关键词 | CELL-GROWTH ; RHO-GTPASE ; TIP GROWTH ; BINDING PROTEINS ; SPATIAL CONTROL ; DYNAMICS ; CYTOSKELETON ; GERMINATION ; POLARITY ; PATHWAYS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000354822800017 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Basic Research Program of China [2012CB114200] ; Natural Science Foundation of China [31361140354] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25589]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Zhenzhen,Shi, Haifan,Chen, Binqing,et al. Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth[J]. PLANT CELL,2015,27(4):1140-1161. |
| APA | Zhou, Zhenzhen,Shi, Haifan,Chen, Binqing,Zhang, Ruihui,Huang, Shanjin,&Fu, Ying.(2015).Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth.PLANT CELL,27(4),1140-1161. |
| MLA | Zhou, Zhenzhen,et al."Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth".PLANT CELL 27.4(2015):1140-1161. |
入库方式： OAI收割
来源：植物研究所
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