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Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza

文献类型：期刊论文

作者Cui, Guanghong1; Duan, Lixin; Jin, Baolong1; Qian, Jun4,5; Xue, Zheyong; Shen, Guoan; Snyder, John Hugh; Song, Jingyuan4,5; Chen, Shilin4,5; Huang, Luqi1
刊名PLANT PHYSIOLOGY
出版日期2015
卷号169期号:3页码:1607-1618
ISSN号0032-0889
DOI10.1104/pp.15.00695
文献子类Article
英文摘要The medicinal plant Salvia miltiorrhiza produces various tanshinone diterpenoids that have pharmacological activities such as vasorelaxation against ischemia reperfusion injury and antiarrhythmic effects. Their biosynthesis is initiated from the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate by sequential reactions catalyzed by copalyl diphosphate synthase (CPS) and kaurene synthase-like cyclases. Here, we report characterization of these enzymatic families from S. miltiorrhiza, which has led to the identification of unique pathways, including roles for separate CPSs in tanshinone production in roots versus aerial tissues (SmCPS1 and SmCPS2, respectively) as well as the unique production of ent-13-epi-manoyl oxide by SmCPS4 and S. miltiorrhiza kaurene synthase-like2 in floral sepals. The conserved SmCPS5 is involved in gibberellin plant hormone biosynthesis. Down-regulation of SmCPS1 by RNA interference resulted in substantial reduction of tanshinones, and metabolomics analysis revealed 21 potential intermediates, indicating a complex network for tanshinone metabolism defined by certain key biosynthetic steps. Notably, the correlation between conservation pattern and stereochemical product outcome of the CPSs observed here suggests a degree of correlation that, especially when combined with the identity of certain key residues, may be predictive. Accordingly, this study provides molecular insights into the evolutionary diversification of functional diterpenoids in plants.
学科主题Plant Sciences
出版地ROCKVILLE
电子版国际标准刊号1532-2548
WOS关键词SINGLE RESIDUE SWITCH ; COPALYL DIPHOSPHATE SYNTHASE ; POSITIVE SELECTION ; ABIETADIENE SYNTHASE ; MAXIMUM-LIKELIHOOD ; HAIRY ROOTS ; BIOSYNTHESIS ; TANSHINONES ; METABOLISM ; CHEMISTRY
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000367393900017
出版者AMER SOC PLANT BIOLOGISTS
资助机构Key Project of Chinese National Programs for Fundamental Research and Development [2013CB127000] ; National Science Fund for Distinguished Young Scholars [81325023] ; National Natural Science Foundation of China [81001604] ; China Academy of Chinese Medical Sciences [ZZ20090301] ; National Institutes of Health [GM076324] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM076324] Funding Source: NIH RePORTER
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25642]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Peters, Reuben J.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA
3.Peking Union Med Coll, Beijing 100193, Peoples R China
4.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China
5.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China
推荐引用方式
GB/T 7714		 Cui, Guanghong,Duan, Lixin,Jin, Baolong,et al. Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza[J]. PLANT PHYSIOLOGY,2015,169(3):1607-1618.
APA Cui, Guanghong.,Duan, Lixin.,Jin, Baolong.,Qian, Jun.,Xue, Zheyong.,...&Qi, Xiaoquan.(2015).Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza.PLANT PHYSIOLOGY,169(3),1607-1618.
MLA Cui, Guanghong,et al."Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza".PLANT PHYSIOLOGY 169.3(2015):1607-1618.

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来源：植物研究所

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