Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube Growth
文献类型：期刊论文
| 作者 | Liu, Xiaonan4; Qu, Xiaolu2,3; Jiang, Yuxiang4; Chang, Ming4; Zhang, Ruihui4; Wu, Youjun4; Fu, Ying; Huang, Shanjin1,3,4 |
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2015 |
| 卷号 | 8期号:12页码:1694-1709 |
| 关键词 | pollen tube actin actin polymerization profilin formin |
| ISSN号 | 1674-2052 |
| DOI | 10.1016/j.molp.2015.09.013 |
| 文献子类 | Article |
| 英文摘要 | Pollen tube growth is an essential step during flowering plant reproduction, whose growth depends on a population of dynamic apical actin filaments. Apical actin filaments were thought to be involved in the regulation of vesicle fusion and targeting in the pollen tube. However, the molecular mechanisms that regulate the construction of apical actin structures in the pollen tube remain largely unclear. Here, we identify profilin as an important player in the regulation of actin polymerization at the apical membrane in the pollen tube. Downregulation of profilin decreased the amount of filamentous actin and induced disorganization of apical actin filaments, and reduced tip-directed vesicle transport and accumulation in the pollen tube. Direct visualization of actin dynamics revealed that the elongation of actin filaments originating at the apical membrane decreased in profilin mutant pollen tubes. Mutant profilin that is defective in binding poly-L-proline only partially rescues the actin polymerization defect in profilin mutant pollen tubes, although it fully rescues the actin turnover phenotype. We propose that profilin controls the construction of actin structures at the pollen tube tip, presumably by favoring formin-mediated actin polymerization at the apical membrane. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1752-9867 |
| WOS关键词 | GROWING PLANT-CELLS ; TIP GROWTH ; FILAMENT ELONGATION ; LILIUM-LONGIFLORUM ; NICOTIANA-TABACUM ; ARP2/3 COMPLEX ; FH1 DOMAIN ; ADP-ACTIN ; IN-VITRO ; ARABIDOPSIS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000366320900002 |
| 出版者 | CELL PRESS |
| 资助机构 | Ministry of Science and Technology of China [2013CB945100, 2011CB944600] ; National Natural Science Foundation of China [31125004, 31121065, 31270223] ; Tsinghua-Peking Joint Center for Life Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25652]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 5.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaonan,Qu, Xiaolu,Jiang, Yuxiang,et al. Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube Growth[J]. MOLECULAR PLANT,2015,8(12):1694-1709. |
| APA | Liu, Xiaonan.,Qu, Xiaolu.,Jiang, Yuxiang.,Chang, Ming.,Zhang, Ruihui.,...&Huang, Shanjin.(2015).Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube Growth.MOLECULAR PLANT,8(12),1694-1709. |
| MLA | Liu, Xiaonan,et al."Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube Growth".MOLECULAR PLANT 8.12(2015):1694-1709. |
入库方式： OAI收割
来源：植物研究所
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