Arabidopsis RZFP34/CHYR1, a Ubiquitin E3 Ligase, Regulates Stomatal Movement and Drought Tolerance via SnRK2.6-Mediated Phosphorylation
文献类型：期刊论文
| 作者 | Ding, Shuangcheng1; Zhang, Bin; Qin, Feng |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2015 |
| 卷号 | 27期号:11页码:3228-3244 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.15.00321 |
| 文献子类 | Article |
| 英文摘要 | Abscisic acid (ABA) is a phytohormone that plays a fundamental role in plant development and stress response, especially in the regulation of stomatal closure in response to water deficit stress. The signal transduction that occurs in response to ABA and drought stress is mediated by protein phosphorylation and ubiquitination. This research identified Arabidopsis thaliana RING ZINC-FINGER PROTEIN34 (RZP34; renamed here as CHY ZINC-FINGER AND RING PROTEIN1 [CHYR1]) as an ubiquitin E3 ligase. CHYR1 expression was significantly induced by ABA and drought, and along with its corresponding protein, was expressed mainly in vascular tissues and stomata. Analysis of CHYR1 gain-of-function and loss-of-function plants revealed that CHYR1 promotes ABA-induced stomatal closure, reactive oxygen species production, and plant drought tolerance. Furthermore, CHYR1 interacted with SNF1-RELATED PROTEIN KINASE2 (SnRK2) kinases and could be phosphorylated by SnRK2.6 on the Thr-178 residue. Overexpression of CHYR1(T178A), a phosphorylation-deficient mutant, interfered with the proper function of CHYR1, whereas CHYR1(T178D) phenocopied the gain of function of CHYR1. Thus, this study identified a RING-type ubiquitin E3 ligase that functions positively in ABA and drought responses and detailed how its ubiquitin E3 ligase activity is regulated by SnRK2.6-mediated protein phosphorylation. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 出版地 | CARY |
| 电子版国际标准刊号 | 1532-298X |
| WOS关键词 | SNRK2 PROTEIN-KINASES ; ABSCISIC-ACID ; HYDROGEN-PEROXIDE ; GENE-EXPRESSION ; TRANSCRIPTION FACTORS ; POSITIVE REGULATOR ; SEED-GERMINATION ; PLANT-RESPONSES ; STRESS ; ABA |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000368295800016 |
| 出版者 | OXFORD UNIV PRESS INC |
| 资助机构 | National Basic Research Program of China [2012CB114302-4] ; National Natural Science Foundation of China [31171163, 31300995] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25761]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Shuangcheng,Zhang, Bin,Qin, Feng. Arabidopsis RZFP34/CHYR1, a Ubiquitin E3 Ligase, Regulates Stomatal Movement and Drought Tolerance via SnRK2.6-Mediated Phosphorylation[J]. PLANT CELL,2015,27(11):3228-3244. |
| APA | Ding, Shuangcheng,Zhang, Bin,&Qin, Feng.(2015).Arabidopsis RZFP34/CHYR1, a Ubiquitin E3 Ligase, Regulates Stomatal Movement and Drought Tolerance via SnRK2.6-Mediated Phosphorylation.PLANT CELL,27(11),3228-3244. |
| MLA | Ding, Shuangcheng,et al."Arabidopsis RZFP34/CHYR1, a Ubiquitin E3 Ligase, Regulates Stomatal Movement and Drought Tolerance via SnRK2.6-Mediated Phosphorylation".PLANT CELL 27.11(2015):3228-3244. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。