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Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.)

文献类型：期刊论文

作者Yan, Shuo1; Wang, Zhongni1; Liu, Yuan; Li, Wei2; Wu, Feng; Lin, Xuelei; Meng, Zheng
刊名PLANT MOLECULAR BIOLOGY
出版日期2015
卷号88期号:4-5页码:415-428
ISSN号0167-4412
关键词Pollen-specific promoter Rice cis-Element Functional architecture Stable transformation
DOI10.1007/s11103-015-0331-0
文献子类Article
英文摘要Late stage pollen-specific promoters are important tools in crop molecular breeding. Several such promoters, and their functional motifs, have been well characterized in dicotyledonous plants such as tomato and tobacco. However, knowledge about the functional architecture of such promoters is limited in the monocotyledonous plant rice. Here, pollen-late-stage-promoter 1 (PLP1) and pollen-late-stage-promoter 2 (PLP2) were characterized using a stable transformation system in rice. Histochemical staining showed that the two promoters exclusively drive GUS expression in late-stage pollen grains in rice. 5' deletion analysis revealed that four regions, including the -1159 to -720 and the -352 to -156 regions of PLP1 and the -740 to -557 and the -557 to -339 regions of PLP2, are important in maintaining the activity and specificity of these promoters. Motif mutation analysis indicated that 'AGAAA' and 'CAAT' motifs in the -740 to -557 region of PLP2 act as enhancers in the promoter. Gain of function experiments indicated that the novel TA-rich motif 'TACATAA' and 'TATTCAT' in the core region of the PLP1 and PLP2 promoters is necessary, but not sufficient, for pollen-specific expression in rice. Our results provide evidence that the enhancer motif 'AGAAA' is conserved in the pollen-specific promoters of both monocots and eudicots, but that some functional architecture characteristics are different.
学科主题Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号1573-5028
出版地DORDRECHT
WOS关键词TISSUE-SPECIFIC EXPRESSION ; CIS-REGULATORY ELEMENTS ; MALE-STERILITY ; TRANSGENIC RICE ; GENE-EXPRESSION ; MESSENGER-RNAS ; PLANTS ; ARABIDOPSIS ; ANTHER ; TOBACCO
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者SPRINGER
WOS记录号WOS:000357052100008
资助机构National Natural Science Foundation of China [31270280] ; Ministry of Science and Technology of China [2011CB100405]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25802]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
3.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China
推荐引用方式
GB/T 7714		 Yan, Shuo,Wang, Zhongni,Liu, Yuan,et al. Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.)[J]. PLANT MOLECULAR BIOLOGY,2015,88(4-5):415-428.
APA Yan, Shuo.,Wang, Zhongni.,Liu, Yuan.,Li, Wei.,Wu, Feng.,...&Meng, Zheng.(2015).Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.).PLANT MOLECULAR BIOLOGY,88(4-5),415-428.
MLA Yan, Shuo,et al."Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.)".PLANT MOLECULAR BIOLOGY 88.4-5(2015):415-428.

入库方式： OAI收割

来源：植物研究所

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