Arabidopsis ACT11 modifies actin turnover to promote pollen germination and maintain the normal rate of tube growth
文献类型：期刊论文
| 作者 | Chang, Ming1; Huang, Shanjin3,4 |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2015 |
| 卷号 | 83期号:3页码:515-527 |
| 关键词 | pollen germination pollen tube growth actin cytoskeleton actin turnover isovariant dynamics |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.12910 |
| 文献子类 | Article |
| 英文摘要 | Actin is an ancient conserved protein that is encoded by multiple isovariants in multicellular organisms. There are eight functional actin genes in the Arabidopsis genome, and the precise function and mechanism of action of each isovariant remain poorly understood. Here, we report the characterization of ACT11, a reproductive actin isovariant. Our studies reveal that loss of function of ACT11 causes a delay in pollen germination, but enhances pollen tube growth. Cytological analysis revealed that the amount of filamentous actin decreased, and the rate of actin turnover increased in act11 pollen. Convergence of actin filaments upon the germination aperture was impaired in act11 pollen, consistent with the observed delay of germination. Reduction of actin dynamics with jasplakinolide suppressed the germination and tube growth phenotypes in act11 pollen, suggesting that the underlying mechanisms involve an increase in actin dynamics. Thus, we demonstrate that ACT11 is required to maintain the rate of actin turnover in order to promote pollen germination and maintain the normal rate of pollen tube growth. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000431390100009 |
| 出版者 | WILEY |
| 资助机构 | Ministry of Science and Technology of China [2013CB945100, 2011CB944600] ; National Natural Science Foundation of China [31125004, 31121065] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25831]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China 3.Tsinghua Univ, Ctr Plant Biol, Sch Life Sci, Beijing 100084, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Ming,Huang, Shanjin. Arabidopsis ACT11 modifies actin turnover to promote pollen germination and maintain the normal rate of tube growth[J]. PLANT JOURNAL,2015,83(3):515-527. |
| APA | Chang, Ming,&Huang, Shanjin.(2015).Arabidopsis ACT11 modifies actin turnover to promote pollen germination and maintain the normal rate of tube growth.PLANT JOURNAL,83(3),515-527. |
| MLA | Chang, Ming,et al."Arabidopsis ACT11 modifies actin turnover to promote pollen germination and maintain the normal rate of tube growth".PLANT JOURNAL 83.3(2015):515-527. |
入库方式： OAI收割
来源：植物研究所
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