Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis
文献类型：期刊论文
| 作者 | Yang, Ke-Zhen; Jiang, Min; Wang, Ming; Xue, Shan; Zhu, Ling-Ling; Wang, Hong-Zhe; Zou, Jun-Jie; Lee, Eun-Kyoung; Sack, Fred10; Le, Jie8
|
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2015 |
| 卷号 | 8期号:5页码:783-795 |
| 关键词 | Arabidopsis stomata development transcription factor phosphorylation |
| ISSN号 | 1674-2052 |
| DOI | 10.1016/j.molp.2014.12.014 |
| 文献子类 | Article |
| 英文摘要 | The initiation of stomatal lineage and subsequent asymmetric divisions in Arabidopsis require the activity of the basic helix-loop-helix transcription factor SPEECHLESS (SPCH). It has been shown that SPCH controls entry into the stomatal lineage as a substrate either of the MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) cascade or GSK3-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2). Here we show that three serine residues of SPCH appear to be the primary phosphorylation targets of Cyclin-Dependent Kinases A; 1 (CDKA; 1) in vitro, and among them Serine 186 plays a crucial role in stomatal formation. Expression of an SPCH construct harboring a mutation that results in phosphorylation deficiencies on Serine 186 residue failed to rescue stomatal defects in spch null mutants. Expression of a phosphorylation-mimic mutant SPCHS186D complemented stomatal production defects in the transgenic lines harboring the targeted expression of dominant-negative CDKA; 1.N146. Therefore, in addition to MAPK-and BIN2-mediated phosphorylation on SPCH, phosphorylation at Serine 186 is positively required for SPCH function in regulating stomatal development. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1752-9867 |
| WOS关键词 | CYCLIN-DEPENDENT-KINASE ; ASYMMETRIC CELL DIVISIONS ; 4 LIPS ; LINEAGE ; DIFFERENTIATION ; PROLIFERATION ; EXPRESSION ; INITIATION ; COMPLEXES ; MUTANTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000353999300010 |
| 出版者 | CELL PRESS |
| 资助机构 | National Natural Science Foundation of China [31271463, 31471362, 31071198, 31471285] ; Chinese Academy of Sciences [KSCX2-YW-N-073] ; Natural Sciences and Engineering Research Council of Canada |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25892]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Wang, Hong-Zhe 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Xue, Shan 4.Wang, Ming 5.Jiang, Min 6.[Yang, Ke-Zhen 7.Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada 8.Zou, Jun-Jie 9.Zhu, Ling-Ling 10.[Lee, Eun-Kyoung |
| 推荐引用方式 GB/T 7714 | Yang, Ke-Zhen,Jiang, Min,Wang, Ming,et al. Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis[J]. MOLECULAR PLANT,2015,8(5):783-795. |
| APA | Yang, Ke-Zhen.,Jiang, Min.,Wang, Ming.,Xue, Shan.,Zhu, Ling-Ling.,...&Le, Jie.(2015).Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis.MOLECULAR PLANT,8(5),783-795. |
| MLA | Yang, Ke-Zhen,et al."Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis".MOLECULAR PLANT 8.5(2015):783-795. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。