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Proteomic analysis of endoplasmic reticulum stress responses in rice seeds

文献类型：期刊论文

作者Qian, Dandan; Tian, Lihong; Qu, Leqing
刊名SCIENTIFIC REPORTS
出版日期2015
卷号5
ISSN号2045-2322
DOI10.1038/srep14255
文献子类Article
英文摘要The defects in storage proteins secretion in the endosperm of transgenic rice seeds often leads to endoplasmic reticulum (ER) stress, which produces floury and shrunken seeds, but the mechanism of this response remains unclear. We used an iTRAQ-based proteomics analysis of ER-stressed rice seeds due to the endosperm-specific suppression of OsSar1 to identify changes in the protein levels in response to ER stress. ER stress changed the expression of 405 proteins in rice seed by > 2.0-fold compared with the wild-type control. Of these proteins, 140 were upregulated and 265 were downregulated. The upregulated proteins were mainly involved in protein modification, transport and degradation, and the downregulated proteins were mainly involved in metabolism and stress/defense responses. A KOBAS analysis revealed that protein-processing in the ER and degradation-related proteasome were the predominant upregulated pathways in the rice endosperm in response to ER stress. Trans-Golgi protein transport was also involved in the ER stress response. Combined with bioinformatic and molecular biology analyses, our proteomic data will facilitate our understanding of the systemic responses to ER stress in rice seeds.
学科主题Multidisciplinary Sciences
出版地LONDON
WOS关键词UNFOLDED PROTEIN RESPONSE ; TRANSCRIPTION FACTOR ; ER STRESS ; QUALITY-CONTROL ; ENDOSPERM CELLS ; GENE-EXPRESSION ; MESSENGER-RNA ; ARABIDOPSIS ; INVOLVEMENT ; PROGLUTELIN
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000361592700001
出版者NATURE PUBLISHING GROUP
资助机构Natural Science Foundation of China [31171368, 31300212]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25929]  
专题中科院植物分子生理学重点实验室
作者单位Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Qian, Dandan,Tian, Lihong,Qu, Leqing. Proteomic analysis of endoplasmic reticulum stress responses in rice seeds[J]. SCIENTIFIC REPORTS,2015,5.
APA Qian, Dandan,Tian, Lihong,&Qu, Leqing.(2015).Proteomic analysis of endoplasmic reticulum stress responses in rice seeds.SCIENTIFIC REPORTS,5.
MLA Qian, Dandan,et al."Proteomic analysis of endoplasmic reticulum stress responses in rice seeds".SCIENTIFIC REPORTS 5(2015).

入库方式： OAI收割

来源：植物研究所

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