Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination
文献类型：期刊论文
| 作者 | Zhao, Qi; Gao, Jing3; Suo, Jinwei3; Chen, Sixue4; Wang, Tai1 ; Dai, Shaojun
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| 刊名 | FRONTIERS IN PLANT SCIENCE
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| 出版日期 | 2015 |
| 卷号 | 6 |
| 关键词 | spore germination Equisetum arvense L. fern proteomics single cell polar growth |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2015.00441 |
| 文献子类 | Article |
| 英文摘要 | Spermatophyte pollen tubes and root hairs have been used as single-cell-type model systems to understand the molecular processes underlying polar growth of plant cells. Horsetail (Equisetum arvense L.) is a perennial herb species in Equisetopsida, which creates separately growing spring and summer stems in its life cycle. The mature chlorophyllous spores produced from spring stems can germinate without dormancy. Here we report the cellular features and protein expression patterns in five stages of horsetail spore germination (mature spores, rehydrated spores, double-celled spores, germinated spores, and spores with protonemal cells). Using 2-DE combined with mass spectrometry, 80 proteins were found to be abundance changed upon spore germination. Among them, proteins involved in photosynthesis, protein turnover, and energy supply were over-represented. Thirteen proteins appeared as proteoforms on the gels, indicating the potential importance of post-translational modification. In addition, the dynamic changes of ascorbate peroxidase, peroxiredoxin, and dehydroascorbate reductase implied that reactive oxygen species homeostasis is critical in regulating cell division and tip-growth. The time course of germination and diverse expression patterns of proteins in photosynthesis, energy supply, lipid and amino acid metabolism indicated that heterotrophic and autotrophic metabolism were necessary in light-dependent germination of the spores. Twenty-six proteins were involved in protein synthesis, folding, and degradation, indicating that protein turnover is vital to spore germination and rhizoid tip-growth. Furthermore, the altered abundance of 14-3-3 protein, small G protein Ran, actin, and caffeoyl-CoA O-methyltransferase revealed that signaling transduction, vesicle trafficking, cytoskeleton dynamics, and cell wall modulation were critical to cell division and polar growth. These findings lay a foundation toward understanding the molecular mechanisms underlying fern spore asymmetric division and rhizoid polar growth. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | POLARIZED CELL-GROWTH ; POLLEN GERMINATION ; SIGNALING PATHWAYS ; ONOCLEA-SENSIBILIS ; CALCIUM CURRENT ; REDUCTASE GENE ; NITRIC-OXIDE ; FERN ; EXPRESSION ; PROTEINS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000357289100001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Natural Science Foundation of China [31071194] ; Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning ; Capacity Construction Project of Local Universities, Shanghai, China [14390502700] ; Funding Program for Young Teachers at Universities and Colleges of Shanghai ; Shanghai Normal University [SK201419] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26030]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Florida, Genet Inst, Interdisciplinary Ctr Biotechnol Res, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL USA 2.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 3.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 4.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Harbin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Qi,Gao, Jing,Suo, Jinwei,et al. Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination[J]. FRONTIERS IN PLANT SCIENCE,2015,6. |
| APA | Zhao, Qi,Gao, Jing,Suo, Jinwei,Chen, Sixue,Wang, Tai,&Dai, Shaojun.(2015).Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination.FRONTIERS IN PLANT SCIENCE,6. |
| MLA | Zhao, Qi,et al."Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination".FRONTIERS IN PLANT SCIENCE 6(2015). |
入库方式： OAI收割
来源：植物研究所
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