Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis
文献类型：期刊论文
| 作者 | Yang, Zhipan; Shang, Zengzhen2; Wang, Lei2 ; Lu, Qingtao; Wen, Xiaogang; Chi, Wei; Zhang, Lixin; Lu, Congming
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| 刊名 | PHOTOSYNTHESIS RESEARCH
 |
| 出版日期 | 2015 |
| 卷号 | 126期号:2-3页码:285-300 |
| 关键词 | Arabidopsis ATase2 Chloroplast development Chloroplast gene expression Purine biosynthesis |
| ISSN号 | 0166-8595 |
| DOI | 10.1007/s11120-015-0131-z |
| 文献子类 | Article |
| 英文摘要 | To investigate the molecular mechanism of chloroplast biogenesis and development, we characterized an Arabidopsis mutant (dg169, delayed greening 169) which showed growth retardation and delayed greening phenotype in leaves. Newly emerged chlorotic leaves recovered gradually with leaf development in the mutant, and the mature leaves showed similar phenotype to those of wild-typewild-type plants. Compared with wild-type, the chloroplasts were oval-shaped and smaller and the thylakoid membranes were less abundant in yellow section of young leaves of dg169. In addition, the functions of photosystem II (PSII) and photosystem I (PSI) were also impaired. Furthermore, the amount of core subunits of PSII and PSI, as well as PSII and PSI complexes reduced in yellow section of young leaves of dg169. Map-based positional cloning identified that phenotype of dg169 was attributed to a point mutation of ATase2 which converts the conserved Ile-155 residue to Asn. ATase2 catalyzes the first step of de novo purine biosynthesis. This mutation resulted in impaired purine synthesis and a significant decrease in ATP, ADP, GTP and GDP contents. The analysis of ATase2-GFP protein fusion showed that ATase2 was localized to nucleoid of chloroplasts. Our results further demonstrated that the levels of PEP-dependent transcripts in yellow section of young leaves of dg169 were decreased while NEP-dependent and both PEP- and NEP-dependent transcripts and chloroplast DNA replications were increased. The results in this study suggest that ATase2 plays an essential role in early chloroplast development through maintaining PEP function. |
| 学科主题 | Plant Sciences |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5079 |
| WOS关键词 | ACTIVE CHROMOSOME COMPLEX ; RNA-POLYMERASE ; PLASTID CHROMOSOME ; LEAF VARIEGATION ; PHOTOSYSTEM-II ; MESSENGER-RNA ; POTATO-TUBERS ; SIGMA FACTORS ; THALIANA ; PROTEIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000362006900009 |
| 出版者 | SPRINGER |
| 资助机构 | State Key Basic Research and Development Plan of China [2015CB150105] ; Chinese Academy of Sciences [KGZD-EW-T05] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25743]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Zhipan,Shang, Zengzhen,Wang, Lei,et al. Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis[J]. PHOTOSYNTHESIS RESEARCH,2015,126(2-3):285-300. |
| APA | Yang, Zhipan.,Shang, Zengzhen.,Wang, Lei.,Lu, Qingtao.,Wen, Xiaogang.,...&Lu, Congming.(2015).Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis.PHOTOSYNTHESIS RESEARCH,126(2-3),285-300. |
| MLA | Yang, Zhipan,et al."Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis".PHOTOSYNTHESIS RESEARCH 126.2-3(2015):285-300. |
入库方式： OAI收割
来源：植物研究所
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