REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis
文献类型：期刊论文
| 作者 | Xu, Gang2; Guo, Haiyan2; Zhang, Dong2; Chen, Dongqin; Jiang, Zhimin2; Lin, Rongcheng
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| 刊名 | PHOTOSYNTHESIS RESEARCH
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| 出版日期 | 2015 |
| 卷号 | 126期号:2-3页码:331-340 |
| 关键词 | Chlorophyll biosynthesis RVE1 POR Seedling greening |
| ISSN号 | 0166-8595 |
| DOI | 10.1007/s11120-015-0146-5 |
| 文献子类 | Article |
| 英文摘要 | Chlorophyll biosynthesis plays a crucial role in the greening process and survival of etiolated seedlings and yet the mechanism underlying the regulation of this process is poorly understood. Upon light stimulation, NADPH: protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide. Whereas this represents a key step in the chlorophyll biosynthetic pathway, the regulation of POR remains largely unknown. Three POR isoforms exist in Arabidopsis thaliana, i.e., PORA, PORB, and PORC. In this study, we identified a transcription factor, REVEILLE1 (RVE1), that binds directly to the PORA promoter through the EE-box cis-regulatory element. Analysis of PORA expression in RVE1 loss-of-function (rve1) and overexpression (RVE1-OX) Arabidopsis plants showed that RVE1 positively regulates the transcription of PORA. We found that Pchlide levels were reduced in RVE1-OX seedlings. Furthermore, rve1 etiolated seedlings had lower greening rates than the wild type when exposed to light, whereas RVE1-OX seedlings had higher greening rates. In addition, when etiolated seedlings were exposed to light, RVE1-OX plants had less reactive oxygen species (ROS) accumulation and cell death than the wild type, and had reduced levels of ROS-responsive gene expression. Taken together, our study reveals an important role for RVE1 in regulating chlorophyll biosynthesis and promoting seedling greening during early plant growth and development. |
| 学科主题 | Plant Sciences |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5079 |
| WOS关键词 | CHLOROPHYLL BIOSYNTHESIS ; REGULATE CHLOROPHYLL ; STRESS RESPONSES ; GENE-EXPRESSION ; SINGLET OXYGEN ; LIGHT ; POR ; THALIANA ; PROTEIN ; DEETIOLATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000362006900013 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China [31170221, 31325002] ; Ministry of Agriculture of China [2014ZX08009-003] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25767]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Gang,Guo, Haiyan,Zhang, Dong,et al. REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis[J]. PHOTOSYNTHESIS RESEARCH,2015,126(2-3):331-340. |
| APA | Xu, Gang,Guo, Haiyan,Zhang, Dong,Chen, Dongqin,Jiang, Zhimin,&Lin, Rongcheng.(2015).REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis.PHOTOSYNTHESIS RESEARCH,126(2-3),331-340. |
| MLA | Xu, Gang,et al."REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis".PHOTOSYNTHESIS RESEARCH 126.2-3(2015):331-340. |
入库方式： OAI收割
来源：植物研究所
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