Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses
文献类型：期刊论文
| 作者 | Jia, Xiao-Hui; Zhang, Peng-Peng; Shi, Ding-Ji; Mi, Hua-Ling; Zhu, Jia-Cheng; Huang, Xi-Wen; He, Pei-Min |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 57期号:5页码:468-476 |
| 关键词 | Anabaena sp PCC 7120 cyclic electron flow pepc gene photosynthesis tolerance |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.12241 |
| 文献子类 | Article |
| 英文摘要 | Since pepc gene encoding phosphoenolpyruvate carboxylase (PEPCase) has been cloned from Anabaena sp. PCC 7120 and other cyanobacteria, the effects of pepc gene expression on photosynthesis have not been reported yet. In this study, we constructed mutants containing either upregulated (forward) or downregulated (reverse) pepc gene in Anabaena sp. PCC 7120. Results from real-time quantitative polymerase chain reaction (RT-qPCR), Western blot and enzymatic analysis showed that PEPCase activity was significantly reduced in the reverse mutant compared with the wild type, and that of the forward mutant was obviously increased. Interestingly, the net photosynthesis in both the reverse mutant and the forward mutant were higher than that of the wild type, but dark respiration was decreased only in the reverse mutant. The absorbance changes of P700 upon saturation pulse showed the photosystem I (PSI) activity was inhibited, as reflected by Y(I), and Y(NA) was elevated, and dark reduction of P700(+) was stimulated, indicating enhanced cyclic electron flow (CEF) around PSI in the reverse mutant. Additionally, the reverse mutant photosynthesis was higher than that of the wild type in low temperature, low and high pH, and high salinity, and this implies increased tolerance in the reverse mutant through downregulated pepc gene. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | PYRIDINE-NUCLEOTIDE DEHYDROGENASE ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; INTERSYSTEM CHAIN ; CHLAMYDOMONAS-REINHARDTII ; NAD(P)H DEHYDROGENASE ; STATE TRANSITIONS ; TRANSPORT ROUTES ; REDOX STATE ; CYANOBACTERIUM ; PHOTOINHIBITION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000353493600002 |
| 出版者 | WILEY |
| 资助机构 | National High-tech R&D Program (863 Program) [2009AA064401, 2014AA093506] ; Chinese Ministry of Environmental Protection [04] ; Shanghai Universities First-class Disciplines Project of Fisheries |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25816]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Shanghai Ocean Univ, Minist Educ Shanghai, Key Lab Explorat & Utilizat Aquat Genet Resource, Shanghai 201306, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Louisiana State Univ, Dep Biol Sci, Baton Rouge, LA 70803 USA 5.[Jia, Xiao-Hui 6.Shi, Ding-Ji 7.Zhu, Jia-Cheng 8.Huang, Xi-Wen |
| 推荐引用方式 GB/T 7714 | Jia, Xiao-Hui,Zhang, Peng-Peng,Shi, Ding-Ji,et al. Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2015,57(5):468-476. |
| APA | Jia, Xiao-Hui.,Zhang, Peng-Peng.,Shi, Ding-Ji.,Mi, Hua-Ling.,Zhu, Jia-Cheng.,...&He, Pei-Min.(2015).Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,57(5),468-476. |
| MLA | Jia, Xiao-Hui,et al."Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 57.5(2015):468-476. |
入库方式： OAI收割
来源：植物研究所
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