Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans
文献类型：期刊论文
| 作者 | Qin, Xiaochun; Wang, Wenda; Chang, Lijing2; Chen, Jinghua; Wang, Peng1; Zhang, Jianping1; He, Yikun2; Kuang, Tingyun; Shen, Jian-Ren
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| 刊名 | PHOTOSYNTHESIS RESEARCH
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| 出版日期 | 2015 |
| 卷号 | 123期号:1页码:61-76 |
| 关键词 | Bryopsis corticulans alpha-Carotene Siphonaxanthin Siphonein Neoxanthin Photosystem I |
| ISSN号 | 0166-8595 |
| DOI | 10.1007/s11120-014-0039-z |
| 文献子类 | Article |
| 英文摘要 | A novel super-complex of photosystem I (PSI)-light-harvesting complex I (LHCI) was isolated from a siphonaceous marine green alga, Bryopsis corticulans. The super-complex contained 9-10 Lhca antennas as external LHCI bound to the core complex. The super-complex was further disintegrated into PSI core and LHCI sub-complexes, and analysis of the pigment compositions by high-performance liquid chromatography revealed unique characteristics of the B. corticulans PSI in that one PSI core contained around 14 a-carotenes and 1-2 e-carotenes. This is in sharp contrast to the PSI core from higher plants and most cyanobacteria where only beta-carotenes were present, and is the first report for an alpha-carotenetype PSI core complex among photosynthetic eukaryotes, suggesting a structural flexibility of the PSI core. Lhca antennas from B. corticulans contained seven kinds of carotenoids (siphonaxanthin, all-trans neoxanthin, 9'-cis neoxanthin, violaxanthin, siphonein, e-carotene, and acarotene) and showed a high carotenoid: chlorophyll ratio of around 7.5: 13. PSI-LHCI super-complex and PSI core showed fluorescence emission peaks at 716 and 718 nm at 77 K, respectively; whereas two Lhca oligomers had fluorescence peaks at 681 and 684 nm, respectively. By comparison with spinach PSI preparations, it was found that B. corticulans PSI had less red chlorophylls, most of them are present in the core complex but not in the outer light-harvesting systems. These characteristics may contribute to the fine tuning of the energy transfer network, and to acclimate to the ever-changing light conditions under which the unique green alga inhabits. |
| 学科主题 | Plant Sciences |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5079 |
| WOS关键词 | LIGHT-HARVESTING-COMPLEX ; PLANT PHOTOSYSTEM-I ; CHLOROPHYLL-PROTEIN COMPLEXES ; CHLAMYDOMONAS-REINHARDTII ; ANGSTROM RESOLUTION ; ENERGY-TRANSFER ; SUPRAMOLECULAR ORGANIZATION ; STARK SPECTROSCOPY ; CRYSTAL-STRUCTURE ; BINDING-PROTEINS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000348379100005 |
| 出版者 | SPRINGER |
| 资助机构 | National Basic Research Program of China (973 Program) [2011CBA00901] ; National Natural Science Foundation [30900095] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25857]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 4.Okayama Univ, Grad Sch Nat Sci & Technol, Photosynth Res Ctr, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Qin, Xiaochun,Wang, Wenda,Chang, Lijing,et al. Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans[J]. PHOTOSYNTHESIS RESEARCH,2015,123(1):61-76. |
| APA | Qin, Xiaochun.,Wang, Wenda.,Chang, Lijing.,Chen, Jinghua.,Wang, Peng.,...&Shen, Jian-Ren.(2015).Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans.PHOTOSYNTHESIS RESEARCH,123(1),61-76. |
| MLA | Qin, Xiaochun,et al."Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans".PHOTOSYNTHESIS RESEARCH 123.1(2015):61-76. |
入库方式： OAI收割
来源：植物研究所
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