Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen
文献类型：期刊论文
| 作者 | Zhang, Jin; Wu, Li-Shuan; Fan, Wei; Zhang, Xiao-Ling; Jia, Hui-Xia; Li, Yu; Yin, Ya-Fang; Hu, Jian-Jun; Lu, Meng-Zhu |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2015 |
| 卷号 | 6 |
| 关键词 | allergen MALDI-TOF/TOF MS/MS pollen Populus deltoids profilin proteomics two-dimensional gel electrophoresis |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2015.00548 |
| 文献子类 | Article |
| 英文摘要 | Proteomic analysis was used to generate a map of Populus deltoides CL. 2KEN8 mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. In addition, the members of profilin allergen family were analyzed and their expression patterns were compared with their homologous genes in Arabidopsis and rice. Knowledge of these identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with poplar pollen allergy. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | CROSS-REACTIVITY ; 2-DIMENSIONAL ELECTROPHORESIS ; HEAT-STRESS ; ARABIDOPSIS ; EXPRESSION ; IDENTIFICATION ; PROFILIN ; REVEALS ; CLASSIFICATION ; AEROALLERGENS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000358726300002 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Special Fund for Forestry Scientific Research in the Public Interest [201304103-4] ; National High-tech R&D Program of China [2011AA100201] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25942]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Fan, Wei 2.[Zhang, Jin 3.Wu, Li-Shuan 4.Zhang, Xiao-Ling 5.Jia, Hui-Xia 6.Li, Yu 7.Hu, Jian-Jun 8.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China 9.Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Jiangsu, Peoples R China 10.Chinese Acad Forestry, Res Inst Wood Ind, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jin,Wu, Li-Shuan,Fan, Wei,et al. Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen[J]. FRONTIERS IN PLANT SCIENCE,2015,6. |
| APA | Zhang, Jin.,Wu, Li-Shuan.,Fan, Wei.,Zhang, Xiao-Ling.,Jia, Hui-Xia.,...&Lu, Meng-Zhu.(2015).Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen.FRONTIERS IN PLANT SCIENCE,6. |
| MLA | Zhang, Jin,et al."Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen".FRONTIERS IN PLANT SCIENCE 6(2015). |
入库方式： OAI收割
来源：植物研究所
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