Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach
文献类型：期刊论文
| 作者 | Rahman, Md. Atikur; Alam, Iftekhar4; Kim, Yong-Goo; Ahn, Na-Young; Heo, Sung-Hyun; Lee, Dong-Gi; Liu, Gongshe3 ; Lee, Byung-Hyun
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| 刊名 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 89页码:112-122 |
| 关键词 | Alfalfa Forage Legume Proteome Salt stress |
| ISSN号 | 0981-9428 |
| DOI | 10.1016/j.plaphy.2015.02.015 |
| 文献子类 | Article |
| 英文摘要 | A comparative proteomic approach was carried out between two contrasting alfalfa cultivars, nonomu (NM-801; salt tolerant) and vernal (VN; salt intolerant) in terms of salt tolerance. Seedlings were subjected to salt stress (50 and 100 mM NaCl) for three days. Several physiological parameters (leaf water, chlorophyll, root Na+, K+, and Ca2+) and root proteome profile were analyzed. Comparison of physiological status revealed that NM-801 is more tolerant to salt than VN. Eighty three differentially expressed proteins were found on 2-DE maps, of which 50 were identified by MALDI-TOF or MALDI-TOF/TOF mass spectrometry. These proteins were involved in ion homeostasis, protein turnover and signaling, protein folding, cell wall components, carbohydrate and energy metabolism, reactive oxygen species regulation and detoxification, and purine and fatty acid metabolism. The comparative proteome analysis showed that 33 salt-responsive proteins were significantly changed in both cultivars, while 17 (14 in VN and 3 in NM-801) were cultivar-specific. Peroxidase, protein disulfide-isomerase, NAD synthetase, and isoflavone reductase were up-regulated significantly only in NM-801 in all salt concentrations. In addition, we identified novel proteins including NAD synthetase and biotin carboxylase-3 that were not reported previously as salt-responsive. Taken together, these results provide new insights of salt stress tolerance in alfalfa. (C) 2015 Elsevier Masson SAS. All rights reserved. |
| 学科主题 | Plant Sciences |
| 出版地 | ISSY-LES-MOULINEAUX |
| 电子版国际标准刊号 | 1873-2690 |
| WOS关键词 | FREE PROLINE ; STRESS ; TOLERANCE ; DROUGHT ; PLANTS ; ROOTS ; IDENTIFICATION ; MECHANISMS ; RESOLUTION ; SEQUENCE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000352331800014 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 资助机构 | National Research Foundation of Korea (NRF) [NRF-2011-616-F00013] ; BK21Plus Program, the Ministry of Education, Republic of Korea |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25583]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Natl Inst Biotechnol, Dhaka 1349, Bangladesh 2.Gyeongsang Natl Univ, PMBBRC, IALS, Div Appl Life Sci BK21Plus, Jinju 660701, South Korea 3.Korea Basic Sci Inst, Div Life Sci, Taejon 305806, South Korea 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Rahman, Md. Atikur,Alam, Iftekhar,Kim, Yong-Goo,et al. Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2015,89:112-122. |
| APA | Rahman, Md. Atikur.,Alam, Iftekhar.,Kim, Yong-Goo.,Ahn, Na-Young.,Heo, Sung-Hyun.,...&Lee, Byung-Hyun.(2015).Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach.PLANT PHYSIOLOGY AND BIOCHEMISTRY,89,112-122. |
| MLA | Rahman, Md. Atikur,et al."Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach".PLANT PHYSIOLOGY AND BIOCHEMISTRY 89(2015):112-122. |
入库方式： OAI收割
来源：植物研究所
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