Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells
文献类型：期刊论文
| 作者 | Wang, Li5,7; Li, Hong5,7; Lv, Xueqin1; Chen, Tong; Li, Ruili1; Xue, Yiqun; Jiang, Jianjun8; Jin, Biao7; Baluska, Frantisek2; Samaj, Jozef3 |
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2015 |
| 卷号 | 8期号:9页码:1334-1349 |
| 关键词 | BRI1 BR signaling endocytosis membrane microdomains spatiotemporal dynamics |
| ISSN号 | 1674-2052 |
| DOI | 10.1016/j.molp.2015.04.005 |
| 文献子类 | Article |
| 英文摘要 | The major brassinosteroid (BR) receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 (BRI1) plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization and assembly state remain unclear. Here, we applied variable angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy to analyze the dynamics of GFP-tagged BRI1. We found that, in response to BR, the degree of co-localization of BRI1-GFP with AtFlot1-mCherry increased, and especially BR stimulated the membrane microdomain-associated pathway of BRI1 internalization. We also verified these observations in endocytosis-defective chc2-1 mutants and the AtFlot1 amiRNA 15-5 lines. Furthermore, examination of the phosphorylation status of bri1-EMS-suppressor 1 and measurement of BR-responsive gene expression revealed that membrane microdomains affect BR signaling. These results suggest that BR promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1752-9867 |
| WOS关键词 | CLATHRIN-INDEPENDENT ENDOCYTOSIS ; CROSS-CORRELATION SPECTROSCOPY ; DETERGENT-RESISTANT MEMBRANES ; SINGLE-MOLECULE ; PLASMA-MEMBRANE ; EGF RECEPTOR ; PLANT-CELLS ; BRASSINOSTEROID RECEPTORS ; DEPENDENT ENDOCYTOSIS ; FLUORESCENT MARKERS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000360980300005 |
| 出版者 | CELL PRESS |
| 资助机构 | 111 project [B13007] ; Major Science Foundation of Ministry of Education of China [313008] ; National Natural Science Foundation of China [91317302, 31271300, 31401149] ; State Key Laboratory of Genetic Engineering, Fudan University ; [ED0007/01/01] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25610]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Peoples R China 2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China 3.Fudan Univ, Sch Life Sci, Inst Plant Biol, Shanghai 200433, Peoples R China 4.Univ Bonn, Inst Cellular & Mol Bot, D-53115 Bonn, Germany 5.Palacky Univ, Dept Cell Biol, Olomouc 78371, Czech Republic 6.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China 7.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 8.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Li,Li, Hong,Lv, Xueqin,et al. Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells[J]. MOLECULAR PLANT,2015,8(9):1334-1349. |
| APA | Wang, Li.,Li, Hong.,Lv, Xueqin.,Chen, Tong.,Li, Ruili.,...&Lin, Jinxing.(2015).Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells.MOLECULAR PLANT,8(9),1334-1349. |
| MLA | Wang, Li,et al."Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells".MOLECULAR PLANT 8.9(2015):1334-1349. |
入库方式： OAI收割
来源：植物研究所
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