TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance
文献类型：期刊论文
| 作者 | Ma, Qing-Hu; Liu, Yun-Chao |
| 刊名 | PLANT MOLECULAR BIOLOGY REPORTER
 |
| 出版日期 | 2015 |
| 卷号 | 33期号:1页码:143-152 |
| 关键词 | Dirigent Lignan Lignin Metabolic flux Pathogen resistance Triticum aestivum |
| ISSN号 | 0735-9640 |
| DOI | 10.1007/s11105-014-0737-x |
| 文献子类 | Article |
| 英文摘要 | Dirigent is proposed to be involved in either lignan or lignin biosynthesis. In the present study, sequences for dirigent and dirigent-like proteins from the wheat genome were analyzed. We obtained wheat dirigent (TaDIR13), and dirigent-like (TaDIR4 and TaJA1) recombinant proteins used for biochemical assays. It was shown that TaDIR13 could effectively direct coniferyl alcohol coupling into (+)-pinoresinol. In contrast, TaDIR4 and TaJA1 did not exhibit this activity. In accordance with this role of TaDIR13, overexpression of TaDIR13 in transgenic tobacco increased total lignan accumulation. Lignan extracts from TaDIR13 transgenic plants had enhanced antibacterial effects to Pseudomonas syringae. Furthermore, these plants did show strong resistance to P. syringae and Phytophthora parasitica. On the contrary, TaDIR4 transgenics did not show noticeable changes in either lignan accumulation or pathogen resistance. Overexpression of TaJA1 did not increase lignan accumulation either. Unlike TaDIR4 plants though, TaJA1 plants exhibited high pathogen resistance; yet, this was achieved through its jacalin-related lectins domain and not its dirigent domain. Lignin content and gene expression were not affected in all transgenic plants. Interestingly, expression of the pinoresinol-lariciresinol reductase gene (NtPLR) was increased in TaDIR13 transgenic plant; this is apparently owing to a favored metabolic flux from coniferyl alcohol into the lignan pathway. These data collectively suggest that TaDIR13 is mainly involved in regulating lignan biosynthesis which, in turn, responsible for the observed roles in pathogen resistance. TaDIR4 and TaJA1, both being dirigent-like proteins, have little functional overlap with dirigent proteins. |
| 学科主题 | Biochemical Research Methods ; Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1572-9818 |
| WOS关键词 | PINORESINOL-LARICIRESINOL REDUCTASE ; CINNAMOYL-COA REDUCTASE ; SPRUCE PICEA SPP. ; GENE-EXPRESSION ; CONIFER DEFENSE ; OVEREXPRESSION ; TRANSCRIPTS ; PHYLOGENY ; DISCOVERY ; DISEASE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000347953500011 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China [31170277, 31370336, 31070235] ; Natural Science Foundation of Beijing [5122024] ; Chinese Academy of Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25621]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.[Ma, Qing-Hu |
| 推荐引用方式 GB/T 7714 | Ma, Qing-Hu,Liu, Yun-Chao. TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(1):143-152. |
| APA | Ma, Qing-Hu,&Liu, Yun-Chao.(2015).TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance.PLANT MOLECULAR BIOLOGY REPORTER,33(1),143-152. |
| MLA | Ma, Qing-Hu,et al."TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance".PLANT MOLECULAR BIOLOGY REPORTER 33.1(2015):143-152. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。