Genome-wide analysis reveals dynamic changes in expression of microRNAs during vascular cambium development in Chinese fir, Cunninghamia lanceolata
文献类型：期刊论文
| 作者 | Qiu, Zongbo4,5; Li, Xiaojuan; Zhao, Yuanyuan; Zhang, Manman5; Wan, Yinglang; Cao, Dechang; Lu, Shanfa1,3; Lin, Jinxing |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2015 |
| 卷号 | 66期号:11页码:3041-3054 |
| 关键词 | Chinese fir (Cunninghamia lanceolata) high-throughput small RNA sequencing microRNA vascular cambium wood formation |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erv103 |
| 文献子类 | Article |
| 英文摘要 | MicroRNAs (miRNAs) are small noncoding regulatory RNAs that play key roles in the process of plant development. To date, extensive studies of miRNAs have been performed in a few model plants, but few efforts have focused on small RNAs (sRNAs) in conifers because of the lack of reference sequences for their enormous genomes. In this study, Solexa sequencing of three sRNA libraries obtained from dormant, reactivating, and active vascular cambium in Chinese.r (Cunninghamia lanceolata) using tangential cryosectioning identified 20 known miRNA families and 18 novel potential miRNAs, of which nine novel miRNA precursors were validated by RT-PCR and sequencing. More than half of these novel miRNAs displayed stage-specific expression patterns in the vascular cambium. Furthermore, analysing the 103 miRNAs and their predicted targets indicated that about 70% appeared to negatively regulate their targets, of which two target genes involved in the regulation of cambial cell division were validated via RNA ligase-mediated rapid amplification of 5'-cDNA ends (RLM 5'-RACE) and transient co-expression in Nicotiana benthamiana leaves. Interestingly, miRNA156 and miRNA172 may regulate the phase transition in vascular cambium from dormancy to active growth. These results provide new insights into the important regulatory functions of miRNAs in vascular cambium development and wood formation in conifers. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | VEGETATIVE PHASE-CHANGE ; GENE-EXPRESSION ; TARGETS ; IDENTIFICATION ; TRANSCRIPTION ; CONSERVATION ; MIRNAS ; MIR156 |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000356225400005 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Programme of Introducing Talents of Discipline to Universities (111 project) [B13007] ; Programme for Changjiang Scholars and Innovative Research Team in University [IRT13047] ; Key Grant Project of Chinese Ministry of Education [313008] ; China Postdoctoral Science Foundation [2012M520457, 2014T70141, 2013M530536] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25758]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Peking Union Med Coll, Beijing 100193, Peoples R China 3.Chinese Acad Med Sci, Med Plant Cultivat Res Ctr, Beijing 100193, Peoples R China 4.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China 5.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Zongbo,Li, Xiaojuan,Zhao, Yuanyuan,et al. Genome-wide analysis reveals dynamic changes in expression of microRNAs during vascular cambium development in Chinese fir, Cunninghamia lanceolata[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(11):3041-3054. |
| APA | Qiu, Zongbo.,Li, Xiaojuan.,Zhao, Yuanyuan.,Zhang, Manman.,Wan, Yinglang.,...&Lin, Jinxing.(2015).Genome-wide analysis reveals dynamic changes in expression of microRNAs during vascular cambium development in Chinese fir, Cunninghamia lanceolata.JOURNAL OF EXPERIMENTAL BOTANY,66(11),3041-3054. |
| MLA | Qiu, Zongbo,et al."Genome-wide analysis reveals dynamic changes in expression of microRNAs during vascular cambium development in Chinese fir, Cunninghamia lanceolata".JOURNAL OF EXPERIMENTAL BOTANY 66.11(2015):3041-3054. |
入库方式： OAI收割
来源：植物研究所
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