MicroRNA profiling analysis throughout tomato fruit development and ripening reveals potential regulatory role of RIN on microRNAs accumulation
文献类型：期刊论文
| 作者 | Gao, Chao; Ju, Zheng; Cao, Dongyan; Zhai, Baiqiang; Qin, Guozheng2 ; Zhu, Hongliang; Fu, Daqi; Luo, Yunbo; Zhu, Benzhong
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| 刊名 | PLANT BIOTECHNOLOGY JOURNAL
 |
| 出版日期 | 2015 |
| 卷号 | 13期号:3页码:370-382 |
| 关键词 | RIN microRNA fruit ripening ethylene high-throughput sequencing tomato |
| ISSN号 | 1467-7644 |
| DOI | 10.1111/pbi.12297 |
| 文献子类 | Article |
| 英文摘要 | The development and ripening of tomato fruit are complex processes involving many gene regulatory pathways at the transcriptional and post-transcriptional level. Ripening inhibitor (RIN) is a vital transcription factor, which targets numerous ripening-related genes at the transcriptional level during tomato fruit ripening. MicroRNAs (miRNAs) are a class of short noncoding RNAs that play important roles in post-transcriptional gene regulation. To elucidate the potential regulatory relationship between rin and miRNAs during fruit development and ripening, we identified known miRNAs and profiled their expression in wild-type tomato and rin mutant using a deep sequencing approach combined with quantitative RT-PCR. A total of 33 known miRNA families were identified, of which 14 miRNA families were differently accumulated. Subsequent promoter analysis showed that possible RIN-binding motifs (CArG-box) tended to occur frequently in the promoter regions of partial differently expressed miRNAs. In addition, ethylene may participate in the regulation of miRNAs accumulation during tomato fruit ripening. Chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay confirmed the direct binding of RIN to the promoter of MIR172a. Collectively, these results showed a close correlation between miRNA expression and RIN as well as ethylene, which further elucidated the regulatory roles of miRNAs during fruit development and ripening and enriched the regulatory network of RIN in tomato fruit. |
| 学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1467-7652 |
| WOS关键词 | SOLANUM-LYCOPERSICON ; DEGRADOME ANALYSIS ; GENETIC-REGULATION ; PLANT MICRORNAS ; READ ALIGNMENT ; SHORT RNAS ; TRANSCRIPTION ; ARABIDOPSIS ; IDENTIFICATION ; EXPRESSION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000352586600009 |
| 出版者 | WILEY |
| 资助机构 | National Natural Sciences Foundation of China [31271959] ; National Basic Research Program ('973' program) of China [2011CB100604] ; Chinese Universities Scientific Fund [2014RC006] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25787]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.China Agr Univ, Coll Food Sci & Nutr Engn, Dept Food Biotechnol, Beijing 100094, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Chao,Ju, Zheng,Cao, Dongyan,et al. MicroRNA profiling analysis throughout tomato fruit development and ripening reveals potential regulatory role of RIN on microRNAs accumulation[J]. PLANT BIOTECHNOLOGY JOURNAL,2015,13(3):370-382. |
| APA | Gao, Chao.,Ju, Zheng.,Cao, Dongyan.,Zhai, Baiqiang.,Qin, Guozheng.,...&Zhu, Benzhong.(2015).MicroRNA profiling analysis throughout tomato fruit development and ripening reveals potential regulatory role of RIN on microRNAs accumulation.PLANT BIOTECHNOLOGY JOURNAL,13(3),370-382. |
| MLA | Gao, Chao,et al."MicroRNA profiling analysis throughout tomato fruit development and ripening reveals potential regulatory role of RIN on microRNAs accumulation".PLANT BIOTECHNOLOGY JOURNAL 13.3(2015):370-382. |
入库方式： OAI收割
来源：植物研究所
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