Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica
文献类型：期刊论文
| 作者 | Li, Yang2; Shi, Lei2
|
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2015 |
| 卷号 | 120期号:2页码:531-538 |
| 关键词 | Osmunda japonica Green spore Maturity level Silica gel desiccation Saturated salt solution desiccation Liquid nitrogen (LN) storage |
| ISSN号 | 0167-6857 |
| DOI | 10.1007/s11240-014-0619-9 |
| 文献子类 | Article |
| 英文摘要 | To improve storage of green spores by liquid nitrogen (LN), we studied the influence of spore maturity and desiccation process on storage of Osmunda japonica spores. Germination percentages of mature spores after drying for 12 h or equilibrating at 10-55 % relative humidity did not significantly differ from those of fresh spores. So we established that the critical water contents of mature spores were about 6.6 and 4.8 % by silica gel and equilibrating with saturated salt solutions, respectively. Germination percentages of post-mature spores after desication were lower than those of fresh spores. The viability of mature spores was always higher than the viability of post-mature spores following varying periods of storage in LN. The germination percentages of mature spores stored in LN after silica gel desication were comparable to those of mature spores desiccated with saturated salt solution, while desiccation with silica gel led to a marked decrease in germination percentages of post-mature spores during subsequent storage. In general, the level of maturity and desiccation process can have an impact on storage of green spores by LN storage. Mature spores can tolerate desiccation and freezing independent of the desication process. However, slow removal of intracellular water led to less damage and provided more protection to post-mature spores during storage. In addition, reduction in water contents of spores to approx. their critical levels can achieve the best results for long-term storage of spores. |
| 学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5044 |
| WOS关键词 | EX-SITU CONSERVATION ; FERN SPORES ; FREEZING SENSITIVITY ; MOISTURE-CONTENT ; ZYGOTIC EMBRYOS ; CRYOPRESERVATION ; TEMPERATURE ; VIABILITY ; SEEDS ; WATER |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000348807200010 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China [31400290] ; National key Technology Support Program of the Ministry of Science and Technology of China [2012BAC01B05] ; Knowledge Innovative Program of The Chinese Academy of Sciences [KSCX2-EW-B-5] ; Special program for technology supporting system of strategic biological resources 'In Vitro Plant Germplasm Collection, BRC-CAS' [CZBZX-1] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25837]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yang,Shi, Lei. Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2015,120(2):531-538. |
| APA | Li, Yang,&Shi, Lei.(2015).Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica.PLANT CELL TISSUE AND ORGAN CULTURE,120(2),531-538. |
| MLA | Li, Yang,et al."Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica".PLANT CELL TISSUE AND ORGAN CULTURE 120.2(2015):531-538. |
入库方式： OAI收割
来源：植物研究所
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