A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis
文献类型：期刊论文
| 作者 | Zhang, Min; Wang, Cuiping; Lin, Qingfang; Liu, Aihua1; Wang, Ting; Feng, Xuanjun; Liu, Jie; Han, Huiling; Ma, Yan; Bonea, Diana2 |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2015 |
| 卷号 | 83期号:4页码:582-599 |
| 关键词 | SSR1 root development auxin transport PIN protein mitochondria |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.12911 |
| 文献子类 | Article |
| 英文摘要 | Auxin polar transport mediated by a group of Pin-formed (PIN) transporters plays important roles in plant root development. However, the mechanism underlying the PIN expression and targeting in response to different developmental and environmental stimuli is still not fully understood. Here, we report a previously uncharacterized gene SSR1, which encodes a mitochondrial protein with tetratricopeptide repeat (TPR) domains, and show its function in root development in Arabidopsis thaliana. In ssr1-2, a SSR1 knock-out mutant, the primary root growth was dramatically inhibited due to severely impaired cell proliferation and cell elongation. Significantly lowered level of auxin was found in ssr1-2 roots by auxin measurement and was further supported by reduced expression of DR5-driven reporter gene. As a result, the maintenance of the root stem cell niche is compromised in ssr1-2. It is further revealed that the expression level of several PIN proteins, namely, PIN1, PIN2, PIN3, PIN4 and PIN7, were markedly reduced in ssr1-2 roots. In particular, we showed that the reduced protein level of PIN2 on cell membrane in ssr1-2 is due to impaired retrograde trafficking, possibly resulting from a defect in retromer sorting system, which destines PIN2 for degradation in vacuoles. In conclusion, our results indicated that SSR1 is functioning in root development in Arabidopsis, possibly by affecting PIN protein expression and subcellular targeting. Significance StatementSSR1, a previous uncharacterized mitochondrial protein, is involved in Arabidopsis root development, possibly by affecting proper PIN protein expression and subcellular targeting. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | STEM-CELL NICHES ; GROWTH ; SCARECROW ; PIN2 ; GRAVITROPISM ; MAINTENANCE ; TRAFFICKING ; HOMEOSTASIS ; REGULATOR ; INTERACTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000359356300002 |
| 出版者 | WILEY |
| 资助机构 | National Nature Science Foundation of China [31170242] ; National Project for Transgenic Research [2014ZX08009-003] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25846]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada 3.Univ Toronto, Dept Biol Sci, Toronto, ON M1C 1A4, Canada |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Wang, Cuiping,Lin, Qingfang,et al. A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis[J]. PLANT JOURNAL,2015,83(4):582-599. |
| APA | Zhang, Min.,Wang, Cuiping.,Lin, Qingfang.,Liu, Aihua.,Wang, Ting.,...&Hua, Xuejun.(2015).A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis.PLANT JOURNAL,83(4),582-599. |
| MLA | Zhang, Min,et al."A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis".PLANT JOURNAL 83.4(2015):582-599. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。