High-throughput screening-compatible assays of As(III) S-adenosylmethionine methyltransferase activity
文献类型：期刊论文
| 作者 | Dong, Hui; Xu, Wenzhong1; Pillai, Jitesh K.; Packianathan, Charles; Rosen, Barry P. |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 480页码:67-73 |
| 关键词 | Arsenic Methylation AS3MT High-throughput screens |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2015.04.011 |
| 文献子类 | Article |
| 英文摘要 | Arsenic is a naturally existing toxin and carcinogen. As(III) S-adenosylmethionine methyltransferases (AS3MT in mammals and ArsM in microbes) methylate As(III) three times in consecutive steps and play a central role in arsenic metabolism from bacteria to humans. Current assays for arsenic methylation are slow, laborious, and expensive. Here we report the development of two in vitro assays for AS3MT activity that are rapid, sensitive, convenient, and relatively inexpensive and can be adapted for high-throughput assays. The first assay measures As(III) binding by the quenching of the protein fluorescence of a single-tryptophan derivative of an AS3MT ortholog. The second assay utilizes time-resolved fluorescence resonance energy transfer to directly measure the conversion of the AS3MT substrate, S-adenosylmethionine, to S-adenosylhomocysteine catalyzed by AS3MT. These two assays are complementary, one measuring substrate binding and the other catalysis, making them useful tools for functional studies and future development of drugs to prevent arsenic-related diseases. (C) 2015 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 出版地 | SAN DIEGO |
| 电子版国际标准刊号 | 1096-0309 |
| WOS关键词 | TOXICITY ; BIOTRANSFORMATION ; BIOMETHYLATION ; IDENTIFICATION ; METHYLATION ; SINEFUNGIN ; MECHANISM ; EXPOSURE ; DISEASE ; TARGET |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000354912300012 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | State of Florida Department of Health [4BF01] ; NIH from the National Institute of Environmental Health Sciences [R01 ES023779] ; Chinese Academy of Sciences ; NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES023779] Funding Source: NIH RePORTER |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25873]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.[Dong, Hui; Pillai, Jitesh K.; Packianathan, Charles; Rosen, Barry P.] Florida Int Univ, Herbert Wertheim Coll Med, Dept Cellular Biol & Pharmacol, Miami, FL 33199 USA 2.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Hui,Xu, Wenzhong,Pillai, Jitesh K.,et al. High-throughput screening-compatible assays of As(III) S-adenosylmethionine methyltransferase activity[J]. ANALYTICAL BIOCHEMISTRY,2015,480:67-73. |
| APA | Dong, Hui,Xu, Wenzhong,Pillai, Jitesh K.,Packianathan, Charles,&Rosen, Barry P..(2015).High-throughput screening-compatible assays of As(III) S-adenosylmethionine methyltransferase activity.ANALYTICAL BIOCHEMISTRY,480,67-73. |
| MLA | Dong, Hui,et al."High-throughput screening-compatible assays of As(III) S-adenosylmethionine methyltransferase activity".ANALYTICAL BIOCHEMISTRY 480(2015):67-73. |
入库方式： OAI收割
来源：植物研究所
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