Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Jiang, Wenbo2; Yin, Qinggang1,2; Wu, Ranran1,2; Zheng, Guangshun1,2; Liu, Jinyue1,2; Dixon, Richard A.; Pang, Yongzhen2 |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2015 |
| 卷号 | 66期号:22页码:7165-7179 |
| 关键词 | Arabidopsis chalcone isomerase-like flavonoids flavonols proanthocyanidins seed coat |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erv413 |
| 文献子类 | Article |
| 英文摘要 | Flavonoids are important natural products for plant defence and human health. Although almost all the flavonoid pathway genes have been well-documented by biochemical and/or genetic approaches, the role of the Arabidopsis chalcone isomerase-like (CHIL) gene remains unclear. Two chil mutants with a seed colour similar to that of wild-type Arabidopsis have been identified here, but in sharp contrast to the characteristic transparent testa seed phenotype associated with other known flavonoid pathway genes. CHIL loss-of-function mutations led to a strong reduction in the proanthocyanidin and flavonol levels in seeds, but not in the anthocyanin levels in leaves. CHIL over-expression could partially recover the mutant phenotype of the chil mutant and increased both proanthocyanidin and flavonol accumulation in wild-type Arabidopsis. However, the CHIL gene could not rescue the mutant phenotype of TT5 that encodes the intrinsic chalcone isomerase in Arabidopsis. Parallel phenotypical and metabolic analyses of the chil, tt5, chs, and f3h mutants revealed that, genetically, CHIL functions at the same step as TT5. Moreover, it is demonstrated that CHIL co-expresses, co-localizes, and interacts with TT5 in Arabidopsis for flavonoid production. Based on these genetic and metabolic studies, it is concluded that CHIL functions with TT5 to promote flavonoid production, which is a unique enhancer in the flavonoid pathway. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | PROANTHOCYANIDIN BIOSYNTHESIS ; MEDICAGO-TRUNCATULA ; SEED ; GENETICS ; ELEMENTS ; MUTANTS ; TESTA ; BIOCHEMISTRY ; ANTHOCYANIN ; MUTAGENESIS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000367815000015 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Major State Basic Research Development Program [2013CB127002] ; National Natural Science Foundation of China [31370335] ; Chinese Academy of Sciences [39391503-7] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25880]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Dixon, Richard A.] Univ N Texas, Dept Biol Sci, Denton, TX USA 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Wenbo,Yin, Qinggang,Wu, Ranran,et al. Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(22):7165-7179. |
| APA | Jiang, Wenbo.,Yin, Qinggang.,Wu, Ranran.,Zheng, Guangshun.,Liu, Jinyue.,...&Pang, Yongzhen.(2015).Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana.JOURNAL OF EXPERIMENTAL BOTANY,66(22),7165-7179. |
| MLA | Jiang, Wenbo,et al."Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana".JOURNAL OF EXPERIMENTAL BOTANY 66.22(2015):7165-7179. |
入库方式： OAI收割
来源：植物研究所
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