Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions
文献类型：期刊论文
| 作者 | Xu, Wenzhong; Dai, Wentao; Yan, Huili; Li, Sheng; Shen, Hongling; Chen, Yanshan; Xu, Hua; Sun, Yangyang; He, Zhenyan; Ma, Mi |
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2015 |
| 卷号 | 8期号:5页码:722-733 |
| ISSN号 | 1674-2052 |
| 关键词 | Arabidopsis arsenite NIP3 1 root-to-shoot translocation tolerance |
| DOI | 10.1016/j.molp.2015.01.005 |
| 文献子类 | Article |
| 英文摘要 | In Arabidopsis, the nodulin 26-like intrinsic protein (NIP) subfamily of aquaporin proteins consists of nine members, five of which (NIP1;1, NIP1; 2, NIP5;1, NIP6;1, and NIP7;1) were previously identified to be permeable to arsenite. However, the roles of NIPs in the root-to-shoot translocation of arsenite in plants remain poorly understood. In this study, using reverse genetic strategies, Arabidopsis NIP3;1 was identified to play an important role in both the arsenic uptake and root-to-shoot distribution under arsenite stress conditions. The nip3;1 loss-of-function mutants displayed obvious improvements in arsenite tolerance for aboveground growth and accumulated less arsenic in shoots than those of the wild-type plants, whereas the nip3; 1 nip1;1 double mutant showed strong arsenite tolerance and improved growth of both roots and shoots under arsenite stress conditions. A promoter-beta-glucuronidase analysis revealed that NIP3; 1 was expressed almost exclusively in roots (with the exception of the root tips), and heterologous expression in the yeast Saccharomyces cerevisiae demonstrated that NIP3; 1 was able to mediate arsenite transport. Taken together, our results suggest that NIP3; 1 is involved in arsenite uptake and root-to-shoot translocation in Arabidopsis, probably as a passive and bidirectional arsenite transporter. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 电子版国际标准刊号 | 1752-9867 |
| 出版地 | CAMBRIDGE |
| WOS关键词 | RICE AQUAPORIN LSI1 ; PHYTOCHELATINS ; ACCUMULATION ; TOLERANCE ; TRANSPORT ; XYLEM ; IDENTIFICATION ; REDUCTION ; PLANTS ; HYPERACCUMULATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000353999300006 |
| 资助机构 | National Natural Science Foundation of China [31170164, 31070449] ; CAS [KSCX2-EW-J-1, KSCX2-YW-G053-4] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25881]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Wenzhong,Dai, Wentao,Yan, Huili,et al. Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions[J]. MOLECULAR PLANT,2015,8(5):722-733. |
| APA | Xu, Wenzhong.,Dai, Wentao.,Yan, Huili.,Li, Sheng.,Shen, Hongling.,...&Ma, Mi.(2015).Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions.MOLECULAR PLANT,8(5),722-733. |
| MLA | Xu, Wenzhong,et al."Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions".MOLECULAR PLANT 8.5(2015):722-733. |
入库方式： OAI收割
来源：植物研究所
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