Comparative De Novo Transcriptome Analysis of Fertilized Ovules in Xanthoceras sorbifolium Uncovered a Pool of Genes Expressed Specifically or Preferentially in the Selfed Ovule That Are Potentially Involved in Late-Acting Self-Incompatibility
文献类型：期刊论文
| 作者 | Zhou, Qingyuan; Zheng, Yuanrun
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2015 |
| 卷号 | 10期号:10 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0140507 |
| 文献子类 | Article |
| 英文摘要 | Xanthoceras sorbifolium, a tree species endemic to northern China, has high oil content in its seeds and is recognized as an important biodiesel crop. The plant is characterized by late-acting self-incompatibility (LSI). LSI was found to occur in many angiosperm species and plays an important role in reducing inbreeding and its harmful effects, as do gametophytic self-incompatibility (GSI) and sporophytic self-incompatibility (SSI). Molecular mechanisms of conventional GSI and SSI have been well characterized in several families, but no effort has been made to identify the genes involved in the LSI process. The present studies indicated that there were no significant differences in structural and histological features between the self-and cross-pollinated ovules during the early stages of ovule development until 5 days after pollination (DAP). This suggests that 5 DAP is likely to be a turning point for the development of the selfed ovules. Comparative de novo transcriptome analysis of the selfed and crossed ovules at 5 DAP identified 274 genes expressed specifically or preferentially in the selfed ovules. These genes contained a significant proportion of genes predicted to function in the biosynthesis of secondary metabolites, consistent with our histological observations in the fertilized ovules. The genes encoding signal transduction-related components, such as protein kinases and protein phosphatases, are over-represented in the selfed ovules. X. sorbifolium selfed ovules also specifically or preferentially express many unique transcription factor (TF) genes that could potentially be involved in the novel mechanisms of LSI. We also identified 42 genes significantly up-regulated in the crossed ovules compared to the selfed ovules. The expression of all 16 genes selected from the RNA-seq data was validated using PCR in the selfed and crossed ovules. This study represents the first genome-wide identification of genes expressed in the fertilized ovules of an LSI species. The availability of a pool of specifically or preferentially expressed genes from selfed ovules for X. sorbifolium will be a valuable resource for future genetic analyses of candidate genes involved in the LSI response. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | LOCUS RECEPTOR KINASE ; PROTEIN-KINASE ; MAP KINASE ; S-LOCUS ; SIGNAL-TRANSDUCTION ; GENOME ANNOTATION ; BREEDING SYSTEM ; ABSCISIC-ACID ; POLLEN ; BRASSICA |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000363028100040 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25946]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qingyuan,Zheng, Yuanrun. Comparative De Novo Transcriptome Analysis of Fertilized Ovules in Xanthoceras sorbifolium Uncovered a Pool of Genes Expressed Specifically or Preferentially in the Selfed Ovule That Are Potentially Involved in Late-Acting Self-Incompatibility[J]. PLOS ONE,2015,10(10). |
| APA | Zhou, Qingyuan,&Zheng, Yuanrun.(2015).Comparative De Novo Transcriptome Analysis of Fertilized Ovules in Xanthoceras sorbifolium Uncovered a Pool of Genes Expressed Specifically or Preferentially in the Selfed Ovule That Are Potentially Involved in Late-Acting Self-Incompatibility.PLOS ONE,10(10). |
| MLA | Zhou, Qingyuan,et al."Comparative De Novo Transcriptome Analysis of Fertilized Ovules in Xanthoceras sorbifolium Uncovered a Pool of Genes Expressed Specifically or Preferentially in the Selfed Ovule That Are Potentially Involved in Late-Acting Self-Incompatibility".PLOS ONE 10.10(2015). |
入库方式： OAI收割
来源：植物研究所
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