中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 广州能源研究所
  3. 中国科学院广州能源研究所
One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling

文献类型：期刊论文

作者Chen, Xiaoyan4; Wang, Fan3; Yu, Qiang2; Liu, Shijie1; Wang, Wen4; Zhang, Yu4; Wang, Zhongming4; Yuan, Zhenhong4
刊名CHEMICAL ENGINEERING SCIENCE
出版日期2022-02-02
卷号248页码:7
关键词D-allullose Dual-enzyme cascade Kinetics stimulation Process optimization
ISSN号0009-2509
DOI10.1016/j.ces.2021.117167
通讯作者Yu, Qiang(yuqiang@ms.giec.ac.cn) ; Liu, Shijie(sliu@esf.edu)
英文摘要One pot cascade of enzymatic catalysis was applied for u-allulose synthesis from ID-glucose by the combination of ID-glucose isomerase (GI) from Escherichia coli MG1655 and u-allulose 3-epimerase (DAE) from Agrobacterium tumefaciens C58. The synthesis process was investigated by characterizing the initial substrate concentration, molar ratios of GI and DAE, the enzyme loading, and contents of cobalt and magnesium ions. The increase of enzyme loading and the molar ratios of GI vs DAE caused the raising of u-allulose yield from 4% to 9%, verifying that the generation rate of D-fructose was an essential factor for rare sugar conversion improvement. The optimal enzyme loadings required for reactions with substrate concentrations of 100 g L-1 and 200 g L-1 were 60 U and 200 U. Kinetics stimulation was conducted to reveal the underlying relationship within the three sugars converted by dual-enzyme, with the fitting curve validated the reliability of the model. (C) 2021 Elsevier Ltd. All rights reserved.
WOS关键词D-PSICOSE 3-EPIMERASE ; D-FRUCTOSE ; ISOMERASE ; TAGATOSE ; MONOSACCHARIDE ; COEXPRESSION ; HYDROGEN ; ENZYME
资助项目National Natural Science Foundation of China[51876206] ; Natural Science Foundation of Guangdong Province[2018A030313012] ; Science and Technology Program of Guangzhou, China[202103000011] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA21050400] ; Young Top-notch Talent of Guangdong Province, China[2016TQ03N647] ; DNL Cooperation Fund, CAS[DNL180305] ; Youth Innovation Promotion Association, CAS[2015289] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081] ; Provincial Science and Technology Project of Guangdong (China)[2017A010105018] ; program of Key Laboratory of Renewable Energy, CAS (China)[E029010801]
WOS研究方向Engineering
语种英语
WOS记录号WOS:000709977400005
出版者PERGAMON-ELSEVIER SCIENCE LTD
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Science and Technology Program of Guangzhou, China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Young Top-notch Talent of Guangdong Province, China ; DNL Cooperation Fund, CAS ; Youth Innovation Promotion Association, CAS ; Municipal Science and Technology Project of Guangzhou (China) ; Provincial Science and Technology Project of Guangdong (China) ; program of Key Laboratory of Renewable Energy, CAS (China)
源URL[http://ir.giec.ac.cn/handle/344007/35139]  
专题中国科学院广州能源研究所
通讯作者Yu, Qiang; Liu, Shijie
作者单位1.SUNY Syracuse, Dept Chem Engn, Coll Environm Sci & Forestry, Syracuse, NY 13210 USA
2.South China Agr Univ, Guangdong Engn Technol Res Ctr Agr & Forestry Bio, Inst Biomass Engn, Key Lab Energy Plants Resource & Utilizat,Minist, Guangzhou 510642, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xiaoyan,Wang, Fan,Yu, Qiang,et al. One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling[J]. CHEMICAL ENGINEERING SCIENCE,2022,248:7.
APA Chen, Xiaoyan.,Wang, Fan.,Yu, Qiang.,Liu, Shijie.,Wang, Wen.,...&Yuan, Zhenhong.(2022).One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling.CHEMICAL ENGINEERING SCIENCE,248,7.
MLA Chen, Xiaoyan,et al."One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling".CHEMICAL ENGINEERING SCIENCE 248(2022):7.

入库方式： OAI收割

来源：广州能源研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。