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Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae

文献类型：期刊论文

作者Zhang, Yi2,3,4,5; Kang, Xihui1; Zhen, Feng3,4,5; Wang, Zhongming4,5; Kong, Xiaoying3,5; Sun, Yongming3,4,5
刊名BIOCHEMICAL ENGINEERING JOURNAL
出版日期2022
卷号177页码:12
ISSN号1369-703X
关键词Enzymatic hydrolysis Disruption Microalgae cell walls Lipid extraction
DOI10.1016/j.bej.2021.108198
通讯作者Wang, Zhongming(wangzm@ms.giec.ac.cn) ; Sun, Yongming(sunym@ms.giec.ac.cn)
英文摘要Enzymatic hydrolysis is emerging as a promising unconventional pretreatment method for disrupting microalgae cells. However, the sustainability of biological enzyme needs to be improved due to low efficiency of enzymatic hydrolysis with single enzyme. Cellulase, xylanase, pectinase, snailase, lysozyme and trypsin were selected to pretreat microalgae Scenedesmus sp. for lipid extraction. The results shown that the pretreatment of a multi-enzyme combination of cellulase, xylanase and pectinase perform a better enzymatic hydrolysis effect than each single enzyme. Results shown that different sequences of enzymatic hydrolysis had a significant effect on the lipid extraction performance from microalgae, the lipid recovery yield of 100% (total lipids content) could be achieved with the sequence of stepwise enzymatic hydrolysis: pectinase (10 mg g(-1) dry weight microalgae), cellulase (20 mg g(-1)) and xylanase (14 mg g(-1)). The SEM image, FTIR measurement, HPLC and XPS analysis indicated that the outer layer of trilaminar sheaths in the microaglal cell walls was first degraded by pectinase, and then cellulose and hemicellulose in the inner layer were destroyed by cellulase and xylanase. This study provides an efficient and environmental-friendly mute for lipid extraction as a new strategy of enzymatic hydrolysis to valorize high-value added products in microalgae downstream processing.
WOS关键词CELL-WALLS ; AMINO-ACID ; EXTRACTION ; HYDROLYSIS ; OPTIMIZATION ; DISRUPTION ; ALGAE ; PRETREATMENT ; CONVERSION ; PRODUCTS
资助项目China and Germany Postdoctoral Exchange Program - Office of the China Postdoctoral Council of the Ministry of Human Resources[ZD2018025] ; Natural Science Foundation of Guangdong Province[2017B020238005] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA21050400] ; Guangzhou Science and Technology Project[201803030007] ; China and Germany Postdoctoral Exchange Program - Helmholtz Association[ZD2018025]
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
语种英语
出版者ELSEVIER
WOS记录号WOS:000717092700001
资助机构China and Germany Postdoctoral Exchange Program - Office of the China Postdoctoral Council of the Ministry of Human Resources ; Natural Science Foundation of Guangdong Province ; Strategic Priority Research Program of Chinese Academy of Sciences ; Guangzhou Science and Technology Project ; China and Germany Postdoctoral Exchange Program - Helmholtz Association
源URL[http://ir.giec.ac.cn/handle/344007/35267]  
专题中国科学院广州能源研究所
通讯作者Wang, Zhongming; Sun, Yongming
作者单位1.Univ Coll Cork, MaREI Ctr, Environm Res Inst, Cork, Ireland
2.Karlsruhe Inst Technol, Inst Pulsed Power & Microwave Technol IHM, D-76344 Eggenstein Leopoldshafen, Germany
3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
4.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
5.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Yi,Kang, Xihui,Zhen, Feng,et al. Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae[J]. BIOCHEMICAL ENGINEERING JOURNAL,2022,177:12.
APA Zhang, Yi,Kang, Xihui,Zhen, Feng,Wang, Zhongming,Kong, Xiaoying,&Sun, Yongming.(2022).Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae.BIOCHEMICAL ENGINEERING JOURNAL,177,12.
MLA Zhang, Yi,et al."Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae".BIOCHEMICAL ENGINEERING JOURNAL 177(2022):12.

入库方式： OAI收割

来源：广州能源研究所

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