Brain iron deficiency and affected contextual fear memory in mice with conditional Ferroportin1 ablation in the brain
文献类型：期刊论文
| 作者 | BSRF用户 |
| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2021 |
| 卷号 | 35期号:2页码:e21174 |
| ISSN号 | 0892-6638 |
| DOI | 10.1096/fj.202000167RR |
| 文献子类 | Article |
| 英文摘要 | Fear memory is a pivotal biological function by which organisms can predict possible danger to avoid or reduce harm. However, dysregulation of fear memory processing may lead to pathological fear or anxiety and produce serious clinical symptoms, such as post-traumatic stress disorder (PTSD). Iron deficiency (ID) is reported to inhibit the initiation of fear memory. In our study, we found that ferroportin1 (FPN1), the only known cellular iron export protein in mammals, and ablation in neurons and astrocytes caused iron deficiency in the cortex and hippocampus. However, little is known about its role in the development of fear memory. Moreover, direct evidence of the role of FPN1, or the related molecular mechanisms of such a role, in balancing brain iron homeostasis, especially in neuronal cells, is lacking. Herein, we deleted Fpn1 in mouse neurons, using Nestin-cre transgenic mice, and explored the impact on neuronal iron recycling and brain iron homeostasis in the cortex and hippocampus. We investigated the response of the mice to contextual fear and found that formation of fear memory was impeded after neuronal FPN1 depletion. We also found that FPN1 ablation in neurons and astrocytes caused an atypical expression of iron metabolism-related proteins in these two regions: decreased expression of DMT1, Ft-H, and Ft-L, and increased TfR1 expression. In addition, the decreased FPN1 in brain microvascular endothelial cells (BMVECs) also shed light on the cause of the decreased iron delivery to the brain through the blood-brain barrier (BBB). Our research highlights the major role played by FPN1 in brain iron homeostasis and identifies a potential target for the treatment of PTSD. |
| 电子版国际标准刊号 | 1530-6860 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000592669300001 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/293143]  |
| 专题 | 北京同步辐射装置 |
| 推荐引用方式 GB/T 7714 | BSRF用户. Brain iron deficiency and affected contextual fear memory in mice with conditional Ferroportin1 ablation in the brain[J]. FASEB JOURNAL,2021,35(2):e21174. |
| APA | BSRF用户.(2021).Brain iron deficiency and affected contextual fear memory in mice with conditional Ferroportin1 ablation in the brain.FASEB JOURNAL,35(2),e21174. |
| MLA | BSRF用户."Brain iron deficiency and affected contextual fear memory in mice with conditional Ferroportin1 ablation in the brain".FASEB JOURNAL 35.2(2021):e21174. |
入库方式： OAI收割
来源：高能物理研究所
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