Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus
文献类型：期刊论文
| 作者 | Ammarah Shabbir4; Wajjiha Batool4; Dan Yu2; Lili Lin4; Qiuli An4; Chen Xiaomin4; Hengyuan Guo2; Shuangshuang Yuan4; Sekete Malota4; Zonghua Wang4 |
| 刊名 | Rice
 |
| 卷号 | 15期号:1 |
| ISSN号 | 1939-8425 |
| 英文摘要 | Endo-β-1,4-Xylanases are a group of extracellular enzymes that catalyze the hydrolysis of xylan, a principal constituent of the plant primary cell wall. The contribution of Endo-β-1,4-Xylanase I to both physiology and pathogenesis of the rice blast fungus M. oryzae is unknown. Here, we characterized the biological function of two endoxylanase I ( MoXYL1A and MoXYL1B ) genes in the development of M. oryzae using targeted gene deletion, biochemical analysis, and fluorescence microscopy. Phenotypic analysis of ? Moxyl1A strains showed that MoXYL1A is required for the full virulence of M. oryzae but is dispensable for the vegetative growth of the rice blast fungus. MoXYL1B , in contrast, did not have a clear role in the infectious cycle but has a critical function in asexual reproduction of the fungus. The double deletion mutant was severely impaired in pathogenicity and virulence as well as asexual development. We found that MoXYL1A deletion compromised appressorium morphogenesis and function, leading to failure to penetrate host cells. Fluorescently tagged MoXYL1A and MoXYL1B displayed cytoplasmic localization in M. oryzae , while analysis of MoXYL1A-GFP and MoXYL1B-GFP in-planta revealed translocation and accumulation of these effector proteins into host cells. Meanwhile, sequence feature analysis showed that MoXYL1A possesses a transient chloroplast targeting signal peptide, and results from an Agrobacterium infiltration assay confirmed co-localization of MoXYL1A-GFP with ChCPN10C-RFP in the chloroplasts of host cells. MoXYL1B, accumulated to the cytoplasm of the host. Taken together, we conclude that MoXYL1A is a secreted effector protein that likely promotes the virulence of M. oryzae by interfering in the proper functioning of the host chloroplast, while the related xylanase MoXYL1B does not have a major role in virulence of M. oryzae . |
| 语种 | 英语 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/174578]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.海南大学 3.闽江学院 4.福建农林大学 |
| 推荐引用方式 GB/T 7714 | Ammarah Shabbir,Wajjiha Batool,Dan Yu,et al. Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus[J]. Rice,15(1). |
| APA | Ammarah Shabbir.,Wajjiha Batool.,Dan Yu.,Lili Lin.,Qiuli An.,...&Justice Norvienyeku. |
| MLA | Ammarah Shabbir,et al."Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus".Rice 15.1 |
入库方式： OAI收割
来源：金属研究所
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