Genome and transcriptome-based characterization of high energy carbon-ion beam irradiation induced delayed flower senescence mutant in Lotus japonicus
文献类型：期刊论文
| 作者 | Du, Yan2,3; Luo, Shanwei2,3,4; Zhao, Jian2,5; Feng, Zhuo2,3; Chen, Xia2,3; Ren, Weibin2,3; Liu, Xiao2,3; Wang, Zhuanzi2; Yu, Lixia2; Li, Wenjian2 |
| 刊名 | BMC PLANT BIOLOGY
 |
| 出版日期 | 2021-11-03 |
| 卷号 | 21期号:1页码:19 |
| 关键词 | Lotus japonicus Flower senescence-delayed Carbon-ion beam irradiation Whole genome re-sequencing RNA-seq Phytohormone |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/s12870-021-03283-0 |
| 通讯作者 | Zhou, Libin(libinzhou@impcas.ac.cn) |
| 英文摘要 | Background Flower longevity is closely related to pollen dispersal and reproductive success in all plants, as well as the commercial value of ornamental plants. Mutants that display variation in flower longevity are useful tools for understanding the mechanisms underlying this trait. Heavy-ion beam irradiation has great potential to improve flower shapes and colors; however, few studies are available on the mutation of flower senescence in leguminous plants. Results A mutant (C416) exhibiting blossom duration eight times longer than that of the wild type (WT) was isolated in Lotus japonicus derived from carbon ion beam irradiation. Genetic assays supported that the delayed flower senescence of C416 was a dominant trait controlled by a single gene, which was located between 4,616,611 Mb and 5,331,876 Mb on chromosome III. By using a sorting strategy of multi-sample parallel genome sequencing, candidate genes were narrowed to the gene CUFF.40834, which exhibited high identity to ethylene receptor 1 in other model plants. A physiological assay demonstrated that C416 was insensitive to ethylene precursor. Furthermore, the dynamic changes of phytohormone regulatory network in petals at different developmental stages was compared by using RNA-seq. In brief, the ethylene, jasmonic acid (JA), and salicylic acid (SA) signaling pathways were negatively regulated in C416, whereas the brassinosteroid (BR) and cytokinin signaling pathways were positively regulated, and auxin exhibited dual effects on flower senescence in Lotus japonicus. The abscisic acid (ABA) signaling pathway is positively regulated in C416. Conclusion So far, C416 might be the first reported mutant carrying a mutation in an endogenous ethylene-related gene in Lotus japonicus, rather than through the introduction of exogenous genes by transgenic techniques. A schematic of the flower senescence of Lotus japonicus from the perspective of the phytohormone regulatory network was provided based on transcriptome profiling of petals at different developmental stages. This study is informative for elucidating the molecular mechanism of delayed flower senescence in C416, and lays a foundation for candidate flower senescence gene identification in Lotus japonicus. It also provides another perspective for the improvement of flower longevity in legume plants by heavy-ion beam. |
| WOS关键词 | PROGRAMMED CELL-DEATH ; POLLINATION-INDUCED ETHYLENE ; CYSTEINE PROTEINASE ; PETAL SENESCENCE ; LEAF SENESCENCE ; ARABIDOPSIS-THALIANA ; SALICYLIC-ACID ; ABSCISIC-ACID ; RECEPTOR GENE ; EXPRESSION |
| 资助项目 | Key-Area Research and Development Program of Guangdong Province[2018B020206002] ; National Natural Science Foundation of China[11705249] ; National Natural Science Foundation of China[11975285] ; National Natural Science Foundation of China[U1632140] ; Youth Innovation Promotion Association of CAS[Y201974] ; Science and Technology Research Cooperation Project of Sichuan Provincial Institutes and Universities[18SYXHZ0014] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000714377700003 |
| 出版者 | BMC |
| 资助机构 | Key-Area Research and Development Program of Guangdong Province ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of CAS ; Science and Technology Research Cooperation Project of Sichuan Provincial Institutes and Universities |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/167507]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Zhou, Libin |
| 作者单位 | 1.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100000, Peoples R China 2.Chinese Acad Sci, Biomed Ctr, Inst Modern Phys, Biophys Grp, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100000, Peoples R China 4.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Guangdong, Peoples R China 5.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730000, Peoples R China 6.Kejin Innovat Inst Heavy Ion Beam Biol Ind, Baiyin 730900, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Yan,Luo, Shanwei,Zhao, Jian,et al. Genome and transcriptome-based characterization of high energy carbon-ion beam irradiation induced delayed flower senescence mutant in Lotus japonicus[J]. BMC PLANT BIOLOGY,2021,21(1):19. |
| APA | Du, Yan.,Luo, Shanwei.,Zhao, Jian.,Feng, Zhuo.,Chen, Xia.,...&Zhou, Libin.(2021).Genome and transcriptome-based characterization of high energy carbon-ion beam irradiation induced delayed flower senescence mutant in Lotus japonicus.BMC PLANT BIOLOGY,21(1),19. |
| MLA | Du, Yan,et al."Genome and transcriptome-based characterization of high energy carbon-ion beam irradiation induced delayed flower senescence mutant in Lotus japonicus".BMC PLANT BIOLOGY 21.1(2021):19. |
入库方式： OAI收割
来源：地理科学与资源研究所
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