CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli
文献类型：期刊论文
| 作者 | Pan, Lang1; Guo, Qiushuang1; Wang, Junzhi1; Shi, Li1; Yang, Xiao2; Zhou, Yaoyu3; Yu, Qin4; Bai, Lianyang1 |
| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2022-04-15 |
| 卷号 | 428页码:13 |
| ISSN号 | 0304-3894 |
| 关键词 | Cyhalofop butyl Metamifop Penoxsulam Cytochrome P450s Herbicide metabolism Methylation analysis O-demethylation |
| DOI | 10.1016/j.jhazmat.2022.128225 |
| 通讯作者 | Zhou, Yaoyu(zhouyy@hunau.edu.cn) ; Yu, Qin(qin.yu@uwa.edu.au) ; Bai, Lianyang(lybai@hunaas.cn) |
| 英文摘要 | Long-term and excessive herbicide use has led to some environmental concerns and especially, herbicide resistance evolution in weeds. Here, we confirmed acetolactate synthase (ALS) inhibiting herbicide penoxsulam resistance and cross resistance to acetyl-coenzyme carboxylase (ACCase) inhibiting herbicides (cyhalofop-butyl and metamifop) in a global weed Echinochloa crus-galli population resistant to these herbicides (R). Penoxsulam metabolism study indicated that degradation rate was significantly higher in R than susceptible E. crus-galli population (S). RNA-sequencing revealed that a cytochrome P450 (P450) gene, CYP81A68, expressed higher in R versus S. Rice seedlings overexpressing this CYP81A68 gene are resistant to penoxsulam, cyhalofop-butyl and metamifop, and penoxsulam resistance is due to enhanced metabolism via O-demethylation. Deletion analysis of the CYP81A68 gene promoter identified an efficient region, in which differential methylation of CpG islands occurred between R and S. Collectively, these results demonstrate that upregulation of E. crus-galli CYP81A68 gene endows generalist metabolic resistance to commonly used ALS- and ACCase-inhibiting herbicides in rice fields and epigenetic regulation may play a role in the resistance evolution. This research could contribute to strategies reducing herbicide environmental impacts by judicious selection of alternative herbicide and nonchemical control tactics. |
| WOS关键词 | RICE CYTOCHROME-P450 GENE ; PEACH-POTATO APHID ; INSECTICIDE RESISTANCE ; DNA METHYLATION ; EXPRESSION ; PENOXSULAM ; METAMIFOP ; MECHANISM ; EVOLUTION ; WEEDS |
| 资助项目 | National Natural Science Foundation of China[32130091] ; National Natural Science Foundation of China[31901905] ; Natural Science Foundation of Hunan Province, China[2021JJ20002] ; Science and Technology Innovation Program of Hunan Province[2021RC3087] ; China Agriculture Research System[CARS-16-E19] ; Australian Grains and Research Development Corporation (GRDC) |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000752475800006 |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Hunan Province, China ; Science and Technology Innovation Program of Hunan Province ; China Agriculture Research System ; Australian Grains and Research Development Corporation (GRDC) |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/170573]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Zhou, Yaoyu; Yu, Qin; Bai, Lianyang |
| 作者单位 | 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China 2.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Land Surface Pattern & Simulat, Beijing 100101, Peoples R China 3.Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China 4.Univ Western Australia, Australian Herbicide Resistance Initiat AHRI, Sch Agr & Environm, Crawley, WA 6009, Australia |
| 推荐引用方式 GB/T 7714 | Pan, Lang,Guo, Qiushuang,Wang, Junzhi,et al. CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli[J]. JOURNAL OF HAZARDOUS MATERIALS,2022,428:13. |
| APA | Pan, Lang.,Guo, Qiushuang.,Wang, Junzhi.,Shi, Li.,Yang, Xiao.,...&Bai, Lianyang.(2022).CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli.JOURNAL OF HAZARDOUS MATERIALS,428,13. |
| MLA | Pan, Lang,et al."CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli".JOURNAL OF HAZARDOUS MATERIALS 428(2022):13. |
入库方式： OAI收割
来源：地理科学与资源研究所
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