In-situ (CO2)-C-13 labeling to trace carbon fluxes in plant-soil-microorganism systems: Review and methodological guideline
文献类型：期刊论文
| 作者 | Pang, Rui3,5; Xu, Xingliang4,5; Tian, Yuqiang6; Cui, Xiaoyong4,7; Ouyang, Hua5; Kuzyakov, Yakov1,2,5,8 |
| 刊名 | RHIZOSPHERE
 |
| 出版日期 | 2021-12-01 |
| 卷号 | 20页码:14 |
| 关键词 | (CO2)-C-13 labeling approach Carbon fluxes Biomarkers Rhizosphere Soil organic carbon |
| DOI | 10.1016/j.rhisph.2021.100441 |
| 通讯作者 | Xu, Xingliang(xuxingl@hotmail.com) |
| 英文摘要 | Carbon (C) cycling in the atmosphere-plant-soil-microorganism system has been the focus of much recent attention, because carbon dioxide (CO2) is the most important greenhouse gas and significantly influences global climate change. It is crucial to understand the fate of newly assimilated C in plants as this process helps clarify complex underground processes, for example, the formation, decomposition, and sequestration of soil organic carbon (SOC), turnover of the microorganisms, and energy flow and matter cycling through the soil food web. Considering the cost effectiveness and feasibility of field operations, pulse labeling plants with C-13 or C-14 via exposure to artificially labeled (CO2)-C-13 or (CO2)-C-14 atmospheres is a more direct and efficient tool, in many cases, the only tool available to investigate C allocation to shoots, roots, and the soil and C utilization by microorganisms. Carbon allocated belowground may be (i) lost to the atmosphere by root respiration, (ii) temporarily stored in the roots, (iii) released in the form of rhizodeposition, (iv) partially stored in microbial biomass, necromass, and soil fauna, (v) respired by rhizosphere microorganisms and soil fauna, or (vi) stored for extended periods as SOC. Rhizodeposition by living roots is a vital C and energy source for rhizosphere microorganisms. Carbon flux in the rhizosphere bridges plants, soil, and microorganisms and modifies microbial SOC decomposition and, therefore, C sequestration. The objectives of this review are to (1) summarize the scope, advantages, and disadvantages of C isotope (C-13 and C-14) applications; (2) propose a protocol for in situ plant (CO2)-C-13 pulse labeling in which all labeling, chasing, sampling, analysis, and calculation steps are described in detail; and (3) discuss the use of combinations of C-13 labeling with biomarkers such as phospholipid fatty acids (PLFAs), amino sugars, nucleic acids (DNA and RNA), and other isotope tracers such as C-14, N-15, and P-33. We believe that the review presents a reliable and standardized protocol for tracing C flow through analyzing isotope enrichment in various pools or biomarkers within atmosphere-plant-soil-microorganism systems. |
| WOS关键词 | RATIO MASS-SPECTROMETRY ; ORGANIC-MATTER SOURCES ; ANIMAL FOOD-WEB ; MICROBIAL BIOMASS ; AMINO-SUGARS ; DELTA-C-13 ANALYSIS ; MURAMIC ACID ; LITTER DECOMPOSITION ; COMMUNITY STRUCTURE ; ASSIMILATED CARBON |
| 资助项目 | National Natural Science Foun-dation of China[U20A2006] ; National Natural Science Foun-dation of China[31988102] ; National Key Research and Development Program of China[2017YFA0604802] ; RUDN University Strategic Academic Leadership Program[075-15-2021-610] |
| WOS研究方向 | Agriculture ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000778819900001 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foun-dation of China ; National Key Research and Development Program of China ; RUDN University Strategic Academic Leadership Program |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/176126]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Xu, Xingliang |
| 作者单位 | 1.RUDN Univ, Agrotechnol Inst, Moscow 117198, Russia 2.Tyumen State Univ, Tyumen 625003, Russia 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 4.Chinese Acad Sci, CAS Ctr Excellence Tibetan Plateau Earth Sci, Beijing 100101, Peoples R China 5.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, 11A Datun Rd, Beijing 100101, Peoples R China 6.Beijing Normal Univ, Fac Geog Sci, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China 7.Univ Chinese Acad Sci, Coll Life Sci, Beijing 101408, Peoples R China 8.Univ Gottingen, Dept Soil Sci Temperate Ecosyst, Dept Agr Soil Sci, Busgenweg 2, D-37077 Gottingen, Germany |
| 推荐引用方式 GB/T 7714 | Pang, Rui,Xu, Xingliang,Tian, Yuqiang,et al. In-situ (CO2)-C-13 labeling to trace carbon fluxes in plant-soil-microorganism systems: Review and methodological guideline[J]. RHIZOSPHERE,2021,20:14. |
| APA | Pang, Rui,Xu, Xingliang,Tian, Yuqiang,Cui, Xiaoyong,Ouyang, Hua,&Kuzyakov, Yakov.(2021).In-situ (CO2)-C-13 labeling to trace carbon fluxes in plant-soil-microorganism systems: Review and methodological guideline.RHIZOSPHERE,20,14. |
| MLA | Pang, Rui,et al."In-situ (CO2)-C-13 labeling to trace carbon fluxes in plant-soil-microorganism systems: Review and methodological guideline".RHIZOSPHERE 20(2021):14. |
入库方式： OAI收割
来源：地理科学与资源研究所
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