Peptide nucleic acid-assisted colorimetric detection of single-nucleotide polymorphisms based on the intrinsic peroxidase-like activity of hemin-carbon nanotube nanocomposites
文献类型：期刊论文
| 作者 | Xu, Mengjia; Xing, Shu; Zhao, Yang; Zhao, Chao |
| 刊名 | TALANTA
 |
| 出版日期 | 2021 |
| 卷号 | 232 |
| 关键词 | STRAND DISPLACEMENT DNA PNA MISMATCHES LIGATION EXTENSION RNA |
| 英文摘要 | Here, taking the advantage of single-stranded (ss) DNA specific nuclease (S1) and peptide nucleic acid (PNA), we demonstrated a novel, rapid, and label-free colorimetric nanosensor for the sensitive and accurate detection of SNPs based on the intrinsic peroxidase-like activity of hemin-functionalized single-walled carbon nanotubes (hemin-SWCNTs). PNA, a man-made mimic of DNA with extraordinary stability toward enzymatic degradation, can effectively protect DNA in the fully matched DNA/PNA duplexes from nuclease digestion. While the DNA in DNA/PNA duplexes containing a mismatch can be cleaved into small fragments. This difference can be visually monitored from the specific color change of TMB/H2O2 system by employing the peroxidase activity of hemin-SWCNTs because of its different aggregation states responding to ssPNA or DNA/PNA duplex. Under optimized conditions, the SNPs in the human tumor suppressor gene TP53 have been successfully genotyped in a linear range of 50-1000 nM with a detection limit of 0.11 nM. Moreover, this platform can effectively discriminate a series of single-base mismatches. This assay avoids the assistance of sophisticated instruments and complicated modifications of probes or nanomaterials, and function well for both cell lysate samples and PCR amplicons from standard cell lines, implying its potential practical applications for bioanalysis and biosensors. |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/21630]  |
| 专题 | 中国科学院宁波材料技术与工程研究所 2021专题_期刊论文 |
| 作者单位 | Zhao, C (corresponding author), Ningbo Univ, Sch Mat Sci & Chem Engn, Ningbo 315211, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Xu, Mengjia,Xing, Shu,Zhao, Yang,et al. Peptide nucleic acid-assisted colorimetric detection of single-nucleotide polymorphisms based on the intrinsic peroxidase-like activity of hemin-carbon nanotube nanocomposites[J]. TALANTA,2021,232. |
| APA | Xu, Mengjia,Xing, Shu,Zhao, Yang,&Zhao, Chao.(2021).Peptide nucleic acid-assisted colorimetric detection of single-nucleotide polymorphisms based on the intrinsic peroxidase-like activity of hemin-carbon nanotube nanocomposites.TALANTA,232. |
| MLA | Xu, Mengjia,et al."Peptide nucleic acid-assisted colorimetric detection of single-nucleotide polymorphisms based on the intrinsic peroxidase-like activity of hemin-carbon nanotube nanocomposites".TALANTA 232(2021). |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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