Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides
文献类型：期刊论文
| 作者 | Wang, Binbin; Liang, Weida; Liang, Hongze; Liu, Bin; Wang, Qiyao; Yan, Yinghua; Wang, Tingting; Jiang, Yufei; Jiang, Yinli; Qiu, Hongda |
| 刊名 | ACS SUSTAINABLE CHEMISTRY & ENGINEERING
 |
| 出版日期 | 2021 |
| 卷号 | 9期号:23页码:7930-7940 |
| 关键词 | HIGHLY SELECTIVE ENRICHMENT PHOSPHOPEPTIDE ENRICHMENT ORGANIC FRAMEWORK RATE CAPABILITY PARTICLES GRAPHENE COMPLEX TAU |
| 英文摘要 | Phosphopeptide enrichment with high selectivity and detection sensitivity is essential for phosphoproteomic studies and remains a long-standing challenge. In this study, new immobilized metal affinity chromatography nanocomposite adsorbents with a phosphonate-functionalized ionic liquid (PFIL) as a surface modifier are successfully prepared via a reaction sequence of amination, quaternization, phosphonate hydrolyzation, and metal immobilization. Taking advantages of integrated features of a flexible and strong tripodal phosphonate chelator, a hydrophilic ionic liquid linker, a large surface area, and the size-exclusion effect, the resulting nanocomposite G@mSiO(2)-PFIL-Ti4+ exhibits excellent detection sensitivity to enrich phosphorylated peptides from a tryptic beta-casein digest (0.15 fmol), and superior enrichment selectivity to capture phosphorylated peptides from a digest mixture of beta-casein and bovine serum albumin (a molar ratio of 1:10,000). Strong immobilization of tripodal chelation to metal ions endows the nanocomposite adsorbent with high tolerance to experimental conditions, and thus excellent reusability of the adsorbent has been achieved without remarkable loss of enrichment efficiency for 10 cycles. Due to the excellent size-exclusion effect, high enrichment specificity of G@mSiO(2)-PFIL-Ti4+ to phosphopeptides has been observed and 23 endogenous phosphopeptides have been captured from human saliva. In addition, 924 phosphopeptides (enrichment specificity, 56.1%) have been identified from the tryptic digest of mouse brain lysate. Particularly, six of 975 phosphorylation sites were Alzheimer's disease-related hyperphosphorylation sites within tau protein. These results demonstrate that G@mSiO(2)-PFIL-Ti4+ nanocomposite affinity materials show great application potential for a proteomic study of complicated biological samples. |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/21834]  |
| 专题 | 中国科学院宁波材料技术与工程研究所 2021专题_期刊论文 |
| 作者单位 | 1.Cui, W (corresponding author), Ningbo Univ, Sch Med, Ningbo Key Lab Behav Neurosci, Zhejiang Prov Key Lab Pathophysiol, Ningbo 315211, Peoples R China. 2.Yan, YH (corresponding author), Ningbo Univ, Key Lab Adv Mass Spectrometry & Mol Anal Zhejiang, Sch Mat Sci & Chem Engn, Ningbo 315211, Peoples R China. 3.Liang, HZ |
| 推荐引用方式 GB/T 7714 | Wang, Binbin,Liang, Weida,Liang, Hongze,et al. Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2021,9(23):7930-7940. |
| APA | Wang, Binbin.,Liang, Weida.,Liang, Hongze.,Liu, Bin.,Wang, Qiyao.,...&Yu, Haibin.(2021).Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,9(23),7930-7940. |
| MLA | Wang, Binbin,et al."Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 9.23(2021):7930-7940. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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