Influence of Hydrogen Bond and Sodium Alginate on Bovine Serum Albumin Adhesion on ZnSe Surface
文献类型:期刊论文
作者 | Zhang Xiao-Xuan; Chu Wu-Bo; Jiang Nan; Li He |
刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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出版日期 | 2021 |
卷号 | 49期号:5页码:E21072-E21078 |
关键词 | SECONDARY STRUCTURE INFRARED-SPECTROSCOPY AQUEOUS ENVIRONMENT PROTEIN ADSORPTION ORIENTATION STABILITY BINDING TRYPSIN |
英文摘要 | Serum albumin is the most abundant protein in mammalian plasma and is often used as a model to understand the adhesion behavior of proteins on surfaces. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) is used to non-destructively and dynamically detect the chemical change information between the protein and the surface during the adhesion process, which is especially suitable for in-situ measurement of samples in water. Therefore, the adhesion of bovine serum albumin (BSA) on the surface in H2O and D2O is investigated by ATR-FTIR technology, which effectively avoids the interference of the characteristic peak of deformation vibration of liquid H2O (1645 cm(-1)) on the amide I band of BSA. Sodium alginate, a polysaccharide widely present in the ocean, is introduced into the BSA solution to explore the changes in the adhesion process of proteins on the surface of ZnSe, and polarized ATR-FTIR spectra are also collected to study the adhesion orientation of BSA. Sodium alginate presents a negative effect on the adhesion of BSA on the surface and there is no specific orientation of BSA on the surface. |
源URL | [http://ir.nimte.ac.cn/handle/174433/21958] ![]() |
专题 | 中国科学院宁波材料技术与工程研究所 2021专题_期刊论文 |
作者单位 | 1.Li, H (corresponding author), Univ Chinese Acad Sci, Ctr Mat Sci & Optoelect Engn, Beijing 100049, Peoples R China. 2.Li, H (corresponding author), Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Key Lab Marine Mat & Related Technol, Zhejiang Key Lab Marine Mat & Protect Technol,CAS, Ningbo 315201, Peoples R China. |
推荐引用方式 GB/T 7714 | Zhang Xiao-Xuan,Chu Wu-Bo,Jiang Nan,et al. Influence of Hydrogen Bond and Sodium Alginate on Bovine Serum Albumin Adhesion on ZnSe Surface[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2021,49(5):E21072-E21078. |
APA | Zhang Xiao-Xuan,Chu Wu-Bo,Jiang Nan,&Li He.(2021).Influence of Hydrogen Bond and Sodium Alginate on Bovine Serum Albumin Adhesion on ZnSe Surface.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,49(5),E21072-E21078. |
MLA | Zhang Xiao-Xuan,et al."Influence of Hydrogen Bond and Sodium Alginate on Bovine Serum Albumin Adhesion on ZnSe Surface".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49.5(2021):E21072-E21078. |
入库方式: OAI收割
来源:宁波材料技术与工程研究所
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