Role of nucleus accumbens microRNA-181a and MeCP2 in incubation of heroin craving in male rats
文献类型：期刊论文
| 作者 | Xu, Wenjin; Hong, Qingxiao; Lin, Zi; Ma, Hong; Chen, Weisheng; Zhuang, Dingding; Zhu, Huaqiang; Lai, Miaojun; Fu, Dan; Zhou, Wenhua |
| 刊名 | PSYCHOPHARMACOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 238期号:8页码:2313-2324 |
| 关键词 | VENTRAL TEGMENTAL AREA TIME-DEPENDENT CHANGES SEEKING BEHAVIOR GENE-EXPRESSION COCAINE INTAKE BDNF CUE WITHDRAWAL EXTINCTION REWARD |
| 英文摘要 | Rationale Epigenetic regulation has been implicated in the incubation of drug craving (the time-dependent increase in drug seeking after prolonged withdrawal from drug self-administration). There is little information available on the role of microRNAs in incubation of heroin craving. Objective This study aimed to investigate the roles and mechanisms of miR-181a and methyl CpG binding protein 2 (MeCP2) in the nucleus accumbens (NAc) in incubation of heroin seeking. Methods MiRNA sequencing was used to predict potential miRNAs, and miRNA profiles were performed in the NAc after 1 day or 14 days after withdrawal from heroin self-administration. Following 14 days of heroin self-administration, rats were injected of lentiviral vectors into the NAc and evaluated for the effects of overexpression of miR-181a or knockdown of MeCP2 on non-reinforced heroin seeking after 14 withdrawal days. Results Lever presses during the heroin-seeking tests were higher after 14 withdrawal days than after 1 day (incubation of heroin craving). miR-181a expression in NAc was lower after 14 withdrawal days than after 1 day, and meCP2 expression in NAc was higher after 14 days than after 1 day. Luciferase activity assay showed that the 3 ' UTR of MeCP2 is directly regulated by miR-181a. Overexpression of miR-181a in NAc decreased heroin seeking after 14 withdrawal days and decreased MeCP2 mRNA and protein expression. Knockdown of MeCP2 expression in NAc by LV-siRNA-MeCP2 also decreased heroin seeking after 14 withdrawal days. Conclusions Results indicate that incubation of heroin craving is mediated in part by time-dependent decreases in NAc miR181a expression that leads to time-dependent increases in MeCP2 expression. Our data suggest that NAc miR-181a and MeCP2 contribute to incubation of heroin craving. |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/21966]  |
| 专题 | 中国科学院宁波材料技术与工程研究所 2021专题_期刊论文 |
| 作者单位 | 1.Zhou, WH 2.Liu, HF (corresponding author), Key Lab Addict Res Zhejiang Prov, Ningbo 315010, Zhejiang, Peoples R China. 3.Liu, HF (corresponding author), Ningbo Univ, Lab Behav Neurosci, Ningbo Kangning Hosp, Ningbo Inst Microcirculat & Henbane,Sch Med, Ningbo 315010, Zhejiang, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Xu, Wenjin,Hong, Qingxiao,Lin, Zi,et al. Role of nucleus accumbens microRNA-181a and MeCP2 in incubation of heroin craving in male rats[J]. PSYCHOPHARMACOLOGY,2021,238(8):2313-2324. |
| APA | Xu, Wenjin.,Hong, Qingxiao.,Lin, Zi.,Ma, Hong.,Chen, Weisheng.,...&Liu, Huifen.(2021).Role of nucleus accumbens microRNA-181a and MeCP2 in incubation of heroin craving in male rats.PSYCHOPHARMACOLOGY,238(8),2313-2324. |
| MLA | Xu, Wenjin,et al."Role of nucleus accumbens microRNA-181a and MeCP2 in incubation of heroin craving in male rats".PSYCHOPHARMACOLOGY 238.8(2021):2313-2324. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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