Dual cascade isothermal amplification reaction based glucometer sensors for point-of-care diagnostics of cancer-related microRNAs
文献类型：期刊论文
| 作者 | Fu, Pan; Xu, Mengjia; Xing, Shu; Zhao, Yang; Zhao, Chao |
| 刊名 | ANALYST
 |
| 出版日期 | 2021 |
| 卷号 | 146期号:10页码:3242-3250 |
| 英文摘要 | The practical use of a point-of-care (POC) device is of particular interest in performing liquid biopsies related to cancer. Herein, taking advantage of the practical convenience of a commercially available personal glucose meter (PGM), we report a convenient, low-cost and sensitive detection strategy for circulating microRNA-155 (miRNA155) in human serum. First, miRNA155 in serum triggers the catalyzed hairpin assembly (CHA) reaction, and then the CHA product is specifically captured by the peptide nucleic acid (PNA) probes attached to the surface of a 96-well plate, which in turn triggers the hybridization chain reaction (HCR), resulting in the local enrichment of invertase. Next, introduction of a substrate (sucrose) for the invertase results in the generation of glucose, which can be detected by a PGM. In this sensor, neutrally charged PNA (12 nt) is more likely to hybridize with the CHA products than with the negatively charged DNA in kinetics, which improves the detection sensitivity and specificity. Due to the synergistic isothermal amplification reaction between CHA and HCR, the sensor is able to achieve a broad dynamic range (from 1 fM to 10 nM) with a detection limit down to 0.36 fM (3 orders of magnitude lower than that without HCR) and is capable of distinguishing single-base mismatched sequences. Thus the convenient, sensitive, robust and low-cost PGM sensor makes on-site nucleic acids detection possible, suggesting its great application prospect as a promising POC device in cancer diagnostics. |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/22083]  |
| 专题 | 中国科学院宁波材料技术与工程研究所 2021专题_期刊论文 |
| 作者单位 | Zhao, C (corresponding author), Ningbo Univ, Sch Mat Sci & Chem Engn, Ningbo 315211, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Fu, Pan,Xu, Mengjia,Xing, Shu,et al. Dual cascade isothermal amplification reaction based glucometer sensors for point-of-care diagnostics of cancer-related microRNAs[J]. ANALYST,2021,146(10):3242-3250. |
| APA | Fu, Pan,Xu, Mengjia,Xing, Shu,Zhao, Yang,&Zhao, Chao.(2021).Dual cascade isothermal amplification reaction based glucometer sensors for point-of-care diagnostics of cancer-related microRNAs.ANALYST,146(10),3242-3250. |
| MLA | Fu, Pan,et al."Dual cascade isothermal amplification reaction based glucometer sensors for point-of-care diagnostics of cancer-related microRNAs".ANALYST 146.10(2021):3242-3250. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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