Akt and its phosphorylation in nucleus accumbens mediate heroin-seeking behavior induced by cues in rats
文献类型：期刊论文
| 作者 | Zhu, Huaqiang; Zhuang, Dingding; Lou, Zhongze; Lai, Miaojun; Fu, Dan; Hong, Qingxiao; Liu, Huifen; Zhou, Wenhua |
| 刊名 | ADDICTION BIOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 26期号:5 |
| 英文摘要 | Akt is initially identified as one of the downstream targets of phosphatidylinositol-3 kinase (PI3K) and is involved in morphine reward and tolerance. However, whether phospholyration of Akt (p-Akt) mediates heroin relapse remains unclear. Here, we aimed to explore the role of p-Akt in the nucleus accumbens (NAc) in cue-induced heroin-seeking behaviors after withdrawal. First, rats were trained to self-administer heroin for 14 days, after which we assessed heroin-seeking behaviors induced by a context cue (CC) or by discrete conditioned cues (CS) after 1 day or 14 days of withdrawal. We found that the active responses induced by CC or CS after 14 days of withdrawal were higher than those after 1 day of withdrawal. Meanwhile, the expression of p-Akt in the NAc was also greatest when rats were exposed to the CS after 14 days of withdrawal. Additionally, a microinjection of LY294002, an inhibitor of PI3K, into the NAc inhibited the CS-induced heroin-seeking behaviors after 14 days of withdrawal, paralleling the decreased levels of p-Akt in the NAc. Finally, Akt1 or beta-arrestin 2 was downregulated via a lentiviral injection to assess the effect on heroin seeking after 14 days of withdrawal. CS-induced heroin-seeking behavior was inhibited by downregulation of Akt1, but not beta-arrestin 2, in the NAc. These data demonstrate that Akt phosphorylation in the NAc may play an important role in the incubation of heroin-seeking behavior, suggesting that the PI3K/Akt pathways may be involved in the process of heroin relapse and addiction. |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/22173]  |
| 专题 | 中国科学院宁波材料技术与工程研究所 2021专题_期刊论文 |
| 作者单位 | Zhou, WH (corresponding author), Ningbo Univ, Sch Med, Ningbo Kangning Hosp, Zhejiang Prov Key Lab Addict Res, Ningbo 315201, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Zhu, Huaqiang,Zhuang, Dingding,Lou, Zhongze,et al. Akt and its phosphorylation in nucleus accumbens mediate heroin-seeking behavior induced by cues in rats[J]. ADDICTION BIOLOGY,2021,26(5). |
| APA | Zhu, Huaqiang.,Zhuang, Dingding.,Lou, Zhongze.,Lai, Miaojun.,Fu, Dan.,...&Zhou, Wenhua.(2021).Akt and its phosphorylation in nucleus accumbens mediate heroin-seeking behavior induced by cues in rats.ADDICTION BIOLOGY,26(5). |
| MLA | Zhu, Huaqiang,et al."Akt and its phosphorylation in nucleus accumbens mediate heroin-seeking behavior induced by cues in rats".ADDICTION BIOLOGY 26.5(2021). |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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