Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen
文献类型：期刊论文
| 作者 | Cheng, Yongfa; Lai, Oi-Ming; Tan, Chin-Ping; Panpipat, Worawan; Cheong, Ling-Zhi; Shen, Cai |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2021 |
| 卷号 | 13期号:3页码:4146-4155 |
| 英文摘要 | Immobilization can be used to improve the stability of lipases and enhances lipase recovery and reusability, which increases its commercial value and industrial applications. Nevertheless, immobilization frequently causes conformational changes of the lipases, which decrease lipase catalytic activity. in the present work, we synthesized UIO-66 and grafted UIO-66 crystals with proline for immobilization of Candida rugosa lipase (CRL). As indicated by steady-state fluorescence microscopy, grafting of proline onto UIO-66 crystals induced beneficial conformational change in CRL. CRL immobilized on UIO-66/Pro (CRL@UIO-66/Pro) demonstrated higher enzyme activity and better recyclability than that immobilized on UIO-66 (CRL@UIO-66) in both hydrolysis (CRL@UIO-66/Pro: 0.34 U; CRL@UIO-66: 0.15 U) and transesterification (CRL@UIO-66/Pro: 0.93 U; CRL@UIO-66: 0.25 U) reactions. The higher values of k(cat) and k(cat)/K-m of CRL@UIO-66/Pro also showed that it had better catalytic efficiency as compared to CRL@UIO-66. It is also worth noting that CRL@UIO-66/Pro (0.93 U) demonstrated a much higher transesterification activity as compared to free CRL (0.11 U), indicating that UIO-66/Pro has increased the solvent stability of CRL. Both CRL@UIO-66 and CRL@UIO-66/Pro were also used for the fabrication of biosensors for nitrofen with a wide linear range (0-100 mu M), lower limit of detection, and good recovery rate. |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/22254]  |
| 专题 | 中国科学院宁波材料技术与工程研究所 2021专题_期刊论文 |
| 作者单位 | 1.Cheong, LZ (corresponding author), Ningbo Univ, Coll Food & Pharmaceut Sci, Dept Food Sci & Engn, Ningbo 315211, Peoples R China. 2.Cheong, LZ (corresponding author), Zhejiang Malaysia Joint Res Lab Agr Prod Proc & N, Ningbo 315211, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Cheng, Yongfa,Lai, Oi-Ming,Tan, Chin-Ping,et al. Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen[J]. ACS APPLIED MATERIALS & INTERFACES,2021,13(3):4146-4155. |
| APA | Cheng, Yongfa,Lai, Oi-Ming,Tan, Chin-Ping,Panpipat, Worawan,Cheong, Ling-Zhi,&Shen, Cai.(2021).Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen.ACS APPLIED MATERIALS & INTERFACES,13(3),4146-4155. |
| MLA | Cheng, Yongfa,et al."Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen".ACS APPLIED MATERIALS & INTERFACES 13.3(2021):4146-4155. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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