Biomimetic self-assembly of lipase-zeolitic imidazolate frameworks with enhanced biosensing of protox inhibiting herbicides
文献类型：期刊论文
| 作者 | Cheng, Chuanchuan; Shen, Cai; Lai, Oi-Ming; Tan, Chin-Ping; Cheong, Ling-Zhi |
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2021 |
| 卷号 | 13期号:42页码:4974-4984 |
| 关键词 | METAL-ORGANIC FRAMEWORKS IMMOBILIZATION NANOPARTICLES BIOCATALYST PESTICIDES NI |
| 英文摘要 | Protox inhibiting herbicides such as nitrofen have detrimental effects on the environment and human health. The current work aims to fabricate a Candida rugosa lipase (CRL)-based electrochemical sensor for rapid and sensitive detection of protox inhibiting herbicides (nitrofen). We proposed the use of poly(vinylpyrrolidone) (PVP) and amino-acids to promote accumulation of Zn2+ ions at the surfaces of Candida rugosa lipase (CRL) and subsequently induce self-assembly of a CRL-zeolitic imidazolate framework (ZIF) structure. This process can be easily and rapidly achieved via a one-pot facile self-assembly method. Steady-state fluorescence spectroscopy indicated that CRL has undergone a conformational change following encapsulation within the ZIF structure. This conformational change is beneficial as the prepared PVP/Glu/CRL@ZIF-8 exhibited enhanced catalytic activity (207% of native CRL), and higher substrate affinity (lower K-m than native CRL) and showed high stability under harsh denaturing conditions. PVP/Glu/CRL@ZIF-8 was finally used for electrochemical biosensing of nitrofen. The fabricated biosensor has a wide linear detection range (0-100 mu M), a lower limit of detection and a good recovery rate. |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/21466]  |
| 专题 | 2021专题_期刊论文 |
| 作者单位 | Cheong, LZ (corresponding author), Ningbo Univ, Coll Food & Pharmaceut Sci, Dept Food Sci, Zhejiang Malaysia Joint Res Lab Agr Prod Proc & N, Ningbo 315211, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Cheng, Chuanchuan,Shen, Cai,Lai, Oi-Ming,et al. Biomimetic self-assembly of lipase-zeolitic imidazolate frameworks with enhanced biosensing of protox inhibiting herbicides[J]. ANALYTICAL METHODS,2021,13(42):4974-4984. |
| APA | Cheng, Chuanchuan,Shen, Cai,Lai, Oi-Ming,Tan, Chin-Ping,&Cheong, Ling-Zhi.(2021).Biomimetic self-assembly of lipase-zeolitic imidazolate frameworks with enhanced biosensing of protox inhibiting herbicides.ANALYTICAL METHODS,13(42),4974-4984. |
| MLA | Cheng, Chuanchuan,et al."Biomimetic self-assembly of lipase-zeolitic imidazolate frameworks with enhanced biosensing of protox inhibiting herbicides".ANALYTICAL METHODS 13.42(2021):4974-4984. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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