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  2. 上海药物研究所
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  4. 新药研究国家重点实验室
Integrating UPLC-HR-MS/MS, Network Pharmacology, and Experimental Validation to Uncover the Mechanisms of Jin'gan Capsules against Breast Cancer

文献类型：期刊论文

作者Qiu, Jianfei2,3,4,5; Zhang, Zhiyin1; Hu, Anling2,3,4,5; Zhao, Peng2,3,4,5; Wei, Xuenai2,3,4,5; Song, Hui2,3,4,5; Yang, Jue2,3,4,5,6,7; Li, Yanmei2,3,4,5,6,7
刊名ACS OMEGA
出版日期2022-08-07
页码13
ISSN号2470-1343
DOI10.1021/acsomega.2c01921
通讯作者Song, Hui(songhui620@126.com) ; Yang, Jue(yaodadewo@163.com) ; Li, Yanmei(liyanmei518@hotmail.com)
英文摘要In the theory of traditional Chinese medicine (TCM), "liver-qi " stagnation and heat-induced toxicity represent the main etiologies of breast cancer. Recently, several TCMs with heat-clearing and detoxification efficacy have shown inhibitory effects on breast cancer. Jin'gan capsules (JGCs), initially approved to treat colds in China, are a heat-clearing and detoxification TCM formula. However, the anticancer activity of JGCs against breast cancer and its underlying mechanisms remain unclear. First, we assessed the antiproliferative activity of JGCs in breast cancer cell lines and evaluated their effects on cell apoptosis and the cell cycle by flow cytometry. Furthermore, we identified the potential bioactive components of JGCs and their corresponding target genes and constructed a bioactive compound-target interaction network by ultra-performance liquid chromatography-high-resolution tandem mass spectrometry (UPLC-HR-MS/MS) and network pharmacology analysis. Finally, the underlying mechanism was investigated through gene function enrichment analysis and experimental validation. We found that JGCs significantly inhibited breast cancer cell growth with IC50 values of 0.56 +/- 0.03, 0.16 +/- 0.03, and 0.94 +/- 0.09 mg/mL for MDA-MB-231, MDA-MB-468, and MCF-7, respectively. In addition, JGC treatment dramatically induced apoptosis and S phase cell cycle arrest in breast cancer cells. Western blot analysis confirmed that JGCs could regulate the protein levels of apoptosis-and cell cycle-related genes. Utilizing UPLC-HR-MS/MS analysis and network pharmacology, we identified 7 potential bioactive ingredients in JGCs and 116 antibreast cancer targets. Functional enrichment analysis indicated that the antitumor effects of JGCs were strongly associated with apoptosis and the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway. Western blot analysis validated that JGC treatment markedly decreased the expression levels of p-JAK2, p-STAT3, and STAT3. Our findings suggest that JGCs suppress breast cancer cell proliferation and induce cell cycle arrest and apoptosis partly by inhibiting the JAK2/STAT3 signaling pathway, highlighting JGCs as a potential therapeutic candidate against breast cancer.
WOS关键词NF-KAPPA-B ; CELL-MIGRATION ; INVASION ; PATHWAY ; NEOANDROGRAPHOLIDE ; ANDROGRAPHOLIDE ; EXPRESSION ; PREDICTION ; APOPTOSIS ; PUERARIN
资助项目National Natural Science Foundation of China ; State Key Laboratory of Drug Research ; Science and Technology Department of Guizhou Province ; 100 Leading Talents of Guizhou Province ; Guizhou Provincial Science and Technology Projects ; Project of Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou ; [82160813] ; [32060210] ; [81872772] ; [81960546] ; [U1812403] ; [SIMM2105KF-15] ; [[2 0 1 8] 1409] ; [[2 0 1 9] 2 7 6 2] ; [[2020] 5008] ; [4Y203] ; [P2018-KF11] ; [QZYY-2019-022] ; [QKHZK [2021] 526] ; [[2021] 448]
WOS研究方向Chemistry
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000844597000001
源URL[http://119.78.100.183/handle/2S10ELR8/302317]  
专题新药研究国家重点实验室
通讯作者Song, Hui; Yang, Jue; Li, Yanmei
作者单位1.Guiyang Hosp, Guizhou Aviat Ind Grp, Guiyang 550025, Peoples R China
2.Guizhou Med Univ, Key Lab Med Mol Biol Guizhou Prov, Guiyang 550004, Peoples R China
3.Guizhou Med Univ, Key Lab Endem & Ethn Dis, Minist Educ, Guiyang 550004, Peoples R China
4.Chinese Acad Sci, Guiyang 550004, Peoples R China
5.Guizhou Med Univ, Key Lab Chem Nat Prod Guizhou Prov, Guiyang 550004, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
7.Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Peoples R China
推荐引用方式
GB/T 7714		 Qiu, Jianfei,Zhang, Zhiyin,Hu, Anling,et al. Integrating UPLC-HR-MS/MS, Network Pharmacology, and Experimental Validation to Uncover the Mechanisms of Jin'gan Capsules against Breast Cancer[J]. ACS OMEGA,2022:13.
APA Qiu, Jianfei.,Zhang, Zhiyin.,Hu, Anling.,Zhao, Peng.,Wei, Xuenai.,...&Li, Yanmei.(2022).Integrating UPLC-HR-MS/MS, Network Pharmacology, and Experimental Validation to Uncover the Mechanisms of Jin'gan Capsules against Breast Cancer.ACS OMEGA,13.
MLA Qiu, Jianfei,et al."Integrating UPLC-HR-MS/MS, Network Pharmacology, and Experimental Validation to Uncover the Mechanisms of Jin'gan Capsules against Breast Cancer".ACS OMEGA (2022):13.

入库方式： OAI收割

来源：上海药物研究所

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