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Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study

文献类型：期刊论文

作者Zhou, Lihong3,4; Ni, Hui2,3; Zhang, Linlin3; Wu, Wenyong2,3; Zhang, Tengqian1,3; Su, Qi2,3; Zhou, Jing3; Long, Huali3; Hou, Jinjun3; Gong, Jiyu4
刊名JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
出版日期2022-08-12
卷号2022页码:10
ISSN号2090-8865
DOI10.1155/2022/8022473
通讯作者Gong, Jiyu(gjy0431@126.com) ; Wu, Wanying(wanyingwu@simm.ac.cn)
英文摘要Objective. Because the response of evaporating light scattering detector (ELSD) being in a nonlinear mode, there is no consensus on the method of calculating its relative correction factors (RCF), which limits the application of the quantitative analysis for multi-components by a single marker (QAMS) with LC-ELSD. Methods. Using eight fructooligosaccharides of Morinda officinalis as a case study, the nystose (GF3) as a single standard was adopted to develop a QAMS method to simultaneously determine the other seven fructooligosaccharides with HILIC-HPLC-ELSD method. Six calculation methods of RCF were investigated to select the most reasonable method. The relative error of content between the QAMS and the external standard method (ESM) obtained from 30 batches of samples was used as an indicator to evaluate the six methods. Finally, a chemometrics analysis was performed to find the differential components among MO and its three processing products. Results. It was first reported that only one calculation method was scientific for calculating RCF for the LC-ELSD method. The RCFs of GF3 to the other seven fructooligosaccharides (GF1-GF8) were obtained as 0.86, 0.91, 0.93, 1.05, 1.15, 1.12, and 1.18, respectively. The QAMS of eight fructooligosaccharides of Morinda officinalis was validated with good linearity (R-2 > 0.9998) and accepted the accuracy of 95-105% (RSD < 1.81%) based on nystose. Finally, Morinda officinalis and its three processed products were distinguished and could be differed based on the content of the eight fructooligosaccharides. Conclusion. The scientific calculation method of RCF would be of great significance for developing the QAMS method in Pharmacopoeia when performing the LC-ELSD method.
WOS关键词RADIX
资助项目Research Project of Science and Technology Commission of Shanghai Municipality[20DZ2201000] ; National Key R&D Program of China[2018YFC1707001] ; National Key R&D Program of China[2018YFC1707900]
WOS研究方向Chemistry
语种英语
出版者HINDAWI LTD
WOS记录号WOS:000843243100001
源URL[http://119.78.100.183/handle/2S10ELR8/302335]  
专题中国科学院上海药物研究所
通讯作者Gong, Jiyu; Wu, Wanying
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210029, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai 201203, Peoples R China
4.Changchun Univ Chinese Med, Coll Pharm, Changchun 130117, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Lihong,Ni, Hui,Zhang, Linlin,et al. Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study[J]. JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY,2022,2022:10.
APA Zhou, Lihong.,Ni, Hui.,Zhang, Linlin.,Wu, Wenyong.,Zhang, Tengqian.,...&Wu, Wanying.(2022).Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study.JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY,2022,10.
MLA Zhou, Lihong,et al."Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study".JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY 2022(2022):10.

入库方式： OAI收割

来源：上海药物研究所

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