Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores
文献类型：期刊论文
| 作者 | Tsoraev, Georgy V. V.7; Protasova, Elena A. A.7; Klimanova, Elizaveta A. A.7; Ryzhykau, Yury L. L.5,6; Kuklin, Alexander I. I.5,6; Semenov, Yury S. S.6; Ge, Baosheng4; Li, Wenjun3 ; Qin, Song4; Friedrich, Thomas2
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| 刊名 | STRUCTURAL DYNAMICS-US
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| 出版日期 | 2022-09-01 |
| 卷号 | 9期号:5页码:11 |
| DOI | 10.1063/4.0000164 |
| 通讯作者 | Maksimov, Eugene G. G.(emaksimoff@yandex.ru) |
| 英文摘要 | The structural organization of natural pigment-protein complexes provides a specific environment for the chromophore groups. Yet, proteins are inherently dynamic and conformationally mobile. In this work, we demonstrate the heterogeneity of chromophores of C-phycocyanin (C-PC) from Arthrospira platensis. Part of the population of trimeric C-PC is subject to spontaneous disturbances of protein-protein interactions resulting in increased conformational mobility of the chromophores. Upon fluorescence excitation in the visible range, the spectral signatures of these poorly populated states are masked by bulk chromophore states, but the former could be clearly discriminated when the fluorescence is excited by near-infrared quanta. Such selective excitation of conformationally mobile C-PC chromophores is due to the structure of their S-1 level, which is characterized by a significantly broadened spectral line. We demonstrate that the anti-Stokes C-PC fluorescence is the result of single-photon absorption. By combining spectral and structural methods, we characterize four distinct states of C-PC chromophores emitting at 620, 650, 665, and 720 nm and assigned the fast component in the anti-Stokes fluorescence decay kinetics in the range of 690-750 nm to the chromophores with increased conformational mobility. Our data suggest that the spectral and temporal characteristics of the anti-Stokes fluorescence can be used to study protein dynamics and develop methods to visualize local environment parameters such as temperature. (C) 2022 Author(s). |
| WOS关键词 | CYANOBACTERIUM SYNECHOCOCCUS-VULCANUS ; ANGLE SCATTERING DATA ; ENERGY-TRANSFER ; THERMAL-STABILITY ; CROSS-SECTION ; PHYCOBILISOME ; PROTEINS ; PHYCOBILIPROTEIN ; ALLOPHYCOCYANIN ; FUNCTIONALITY |
| WOS研究方向 | Chemistry ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000849330000001 |
| 资助机构 | Russian Science Foundation ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province, China ; Ministry of Science and Higher Education of the Russian Federation |
| 源URL | [http://ir.yic.ac.cn/handle/133337/31655]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Maksimov, Eugene G. G. |
| 作者单位 | 1.Russian Acad Sci, Fed Res Ctr Biotechnol, AN Bach Inst Biochem, Moscow 119071, Russia 2.Tech Univ Berlin, Inst Chem PC 14, D-10623 Berlin, Germany 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.China Univ Petr Huadong, Coll Chem Engn, Qingdao 266580, Peoples R China 5.Joint Inst Nucl Res, Frank Lab Neutron Phys, Dubna 141980, Russia 6.Moscow Inst Phys & Technol, Res Ctr Mol Mech Aging & Age Related Dis, Dolgoprudnyi 141700, Russia 7.Lomonosov Moscow State Univ, Fac Biol, Moscow 119991, Russia |
| 推荐引用方式 GB/T 7714 | Tsoraev, Georgy V. V.,Protasova, Elena A. A.,Klimanova, Elizaveta A. A.,et al. Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores[J]. STRUCTURAL DYNAMICS-US,2022,9(5):11. |
| APA | Tsoraev, Georgy V. V..,Protasova, Elena A. A..,Klimanova, Elizaveta A. A..,Ryzhykau, Yury L. L..,Kuklin, Alexander I. I..,...&Maksimov, Eugene G. G..(2022).Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores.STRUCTURAL DYNAMICS-US,9(5),11. |
| MLA | Tsoraev, Georgy V. V.,et al."Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores".STRUCTURAL DYNAMICS-US 9.5(2022):11. |
入库方式： OAI收割
来源：烟台海岸带研究所
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